Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance

Tuberculosis (TB) is a disease instigated by Mycobacterium tuberculosis . Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of M. tuberculosis drug resistance i...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2021-06, Vol.52 (2), p.607-617
Main Authors: Shahzad, Faheem, Bashir, Noman, Ali, Atia, Jabeen, Shagufta, Kashif, Mohammad, Javaid, Khursheed, Tahir, Romeeza, Abbas, Afia, Jahan, Shah, Afzal, Nadeem
Format: Article
Language:English
Subjects:
Adult
Antigens, CD - blood
Antigens, CD - economics
Antigens, Differentiation, Myelomonocytic - blood
Antigens, Differentiation, Myelomonocytic - economics
Antitubercular Agents - pharmacology
Biomedical and Life Sciences
CD14 antigen
CD16 antigen
CD163 antigen
Clinical Microbiology - Research Paper
Drug resistance
Drug Resistance, Bacterial
Effector cells
Female
Flow cytometry
Food Microbiology
Humans
Innate immunity
Life Sciences
Male
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Middle Aged
Monocytes
Monocytes - metabolism
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - physiology
Mycology
Peripheral blood
Receptors, Cell Surface - blood
Rifampin
Rifampin - pharmacology
Tuberculosis
Tuberculosis - blood
Tuberculosis - drug therapy
Tuberculosis - microbiology
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Summary:Tuberculosis (TB) is a disease instigated by Mycobacterium tuberculosis . Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of M. tuberculosis drug resistance in patients with TB. This study was conducted to investigate alterations in monocyte subsets, CD163 expression on monocytes, and its serum level in patients without and with rifampicin resistance TB (RR-TB) and healthy controls. A total of 164 patients with TB (84 without RR-TB and 80 patients with RR-TB) and 85 healthy controls were enrolled in this study. The percentages of various monocyte subsets and surface expression of CD163 on monocytes were quantitatively determined using flow cytometry. The serum level of CD163 was determined by commercially available ELISA kits. Decreased frequency of classical monocytes was detected in patients with RR-TB. Non-classical monocytes were decreased in patients without RR-TB; however, intermediate monocytes were raised in patients with RR-TB. The serum level of CD163 was decreased in patients of RR-TB that showsed a positive correlation with the frequency of CD14 ++ CD16 – CD163 + and CD14 ++ CD16 + CD163 + monocytes. It is concluded that decreased classical monocytes and sCD163 in patients with RR-TB could be an indicator of drug resistance.
ISSN:1517-8382
1678-4405
DOI:10.1007/s42770-021-00454-x