Intercellular bridges coordinate the transition from pluripotency to meiosis in mouse fetal oocytes

Meiosis is critical to generating oocytes and ensuring female fertility; however, the mechanisms regulating the switch from mitotic primordial germ cells to meiotic germ cells are poorly understood. Here, we implicate intercellular bridges (ICBs) in this state transition. We used three-dimensional i...

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Bibliographic Details
Published in:Science advances 2021-04, Vol.7 (15)
Main Authors: Soygur, B, Jaszczak, R G, Fries, A, Nguyen, D H, Malki, S, Hu, G, Demir, N, Arora, R, Laird, D J
Format: Article
Language:English
Subjects:
Animals
Cell Biology
Chromosomal Proteins, Non-Histone
Developmental Biology
Female
Fetus
Germ Cells
Meiosis
Mice
Oocytes
Ovary
SciAdv r-articles
Transcription Factors - genetics
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Summary:Meiosis is critical to generating oocytes and ensuring female fertility; however, the mechanisms regulating the switch from mitotic primordial germ cells to meiotic germ cells are poorly understood. Here, we implicate intercellular bridges (ICBs) in this state transition. We used three-dimensional in toto imaging to map meiotic initiation in the mouse fetal ovary and revealed a radial geometry of this transition that precedes the established anterior-posterior wave. Our studies reveal that appropriate timing of meiotic entry across the ovary and coordination of mitotic-meiotic transition within a cyst depend on the ICB component , which we show is required for functional cytoplasmic sharing. We find that mutants more rapidly attenuate the pluripotency transcript upon meiotic initiation, and mutants undergo premature meiosis similar to Together, these results lead to a model that ICBs coordinate and buffer the transition from pluripotency to meiosis through dilution of regulatory factors.
ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.abc6747