Trop2 is upregulated in the transition to dysplasia in the metaplastic gastric mucosa

Intestinal‐type gastric adenocarcinoma arises in a field of pre‐existing metaplasia. While biomarkers of cancer and metaplasia have been identified, the definition of dysplastic transition as a critical point in the evolution of cancer has remained obscure. We have evaluated Trop2 as a putative mark...

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Bibliographic Details
Published in:The Journal of pathology 2020-07, Vol.251 (3), p.336-347
Main Authors: Riera, Katherine M, Jang, Bogun, Min, Jimin, Roland, Joseph T, Yang, Qing, Fesmire, William T, Camilleri‐Broet, Sophie, Ferri, Lorenzo, Kim, Woo H, Choi, Eunyoung, Goldenring, James R
Format: Article
Language:English
Subjects:
Adenocarcinoma
Animal models
Animals
Antigens, Neoplasm - genetics
Antigens, Neoplasm - metabolism
Basic Helix-Loop-Helix Transcription Factors - genetics
CD44 variant 9
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - metabolism
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - metabolism
Cell Transformation, Neoplastic - pathology
Cells, Cultured
Disease Models, Animal
Dysplasia
Gastric cancer
Gastric mucosa
Gastric Mucosa - metabolism
Gastric Mucosa - pathology
Genes, ras
Glands
Humans
intestinal metaplasia
Intestine
Metaplasia
Mice, Transgenic
Microfilament Proteins - genetics
Microfilament Proteins - metabolism
Organoids
Precancerous Conditions - genetics
Precancerous Conditions - metabolism
Precancerous Conditions - pathology
Signal Transduction
SPEM
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Tacstd2
Up-Regulation
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Summary:Intestinal‐type gastric adenocarcinoma arises in a field of pre‐existing metaplasia. While biomarkers of cancer and metaplasia have been identified, the definition of dysplastic transition as a critical point in the evolution of cancer has remained obscure. We have evaluated Trop2 as a putative marker of the transition from metaplasia to dysplasia in the stomach in multiple mouse models of metaplasia induction and progression. In addition, TROP2 expression was evaluated in human samples by immunostaining tissue microarrays for metaplasia, dysplasia, and gastric cancer. Dysplastic mouse organoids were evaluated in vitro following shRNA knockdown of Trop2 expression. In mouse models, no Trop2 was observed in the normal corpus and Trop2 was not induced in acute models of metaplasia induction with either L635 or DMP‐777. In Mist1‐Kras mice, Trop2 expression was not observed in metaplasia at 1 month after Kras induction, but was observed in dysplastic glands at 3–4 months after Kras induction. In human tissues, no Trop2 was observed in normal corpus mucosa or SPEM, but Trop2 expression was observed in incomplete intestinal metaplasia, with significantly less expression in complete intestinal metaplasia. Trop2 expression was observed in all dysplastic and 84% of gastric cancer lesions, although expression levels were variable. Dysplastic mouse organoids from Mist1‐Kras mice expressed Trop2 strongly. Knockdown of Trop2 with shRNA markedly reduced organoid growth and budding behavior, and induced the upregulation of apical villin expression. We conclude that Trop2 is upregulated in the transition to dysplasia in the stomach and promotes dysplastic cell behaviors. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
ISSN:0022-3417
1096-9896
DOI:10.1002/path.5469