Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase

A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplant...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2021-05, Vol.496 (1), p.5-9
Main Authors: Beilin, A. K., Gurskaya, N. G., Evtushenko, N. A., Alpeeva, E. V., Kosykh, A. V., Terskikh, V. V., Vasiliev, A. V., Vorotelyak, E. A.
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biochemistry, Biophysics, and Molecular Biology
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Catalytic Domain
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - metabolism
Cell Transformation, Neoplastic - pathology
Chromosome Aberrations
Contact inhibition
Cytology
Heterografts
Humans
Immortalization
Keratinocytes
Keratinocytes - cytology
Keratinocytes - enzymology
Keratinocytes - metabolism
Life Sciences
Male
Mice
Mice, Nude
Molecular Biology
Primary Cell Culture
Telomerase
Telomerase - genetics
Telomerase - metabolism
Transplantation
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Description
Summary:A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplantation into nude mice the cells have tumorigenic properties.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672921010014