In vitro screening as an anthelmintic discovery pipeline for Calicophoron daubneyi: nutritive media and rumen environment-based approaches

Paramphistomosis can lead to morbidity and mortality of ruminant livestock within tropical and sub-tropical climates. In recent decades, rumen fluke has become an emerging infection in temperate climates across Western Europe, with Calicophoron daubneyi , the primary species present. Clinical outbre...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology research (1987) 2021-04, Vol.120 (4), p.1351-1362
Main Authors: Huson, K. M., Morphew, R. M., Winters, A., Cookson, A., Hauck, B., Brophy, P. M.
Format: Article
Language:English
Subjects:
Animals
Anthelmintic agents
Antiplatyhelmintic Agents - pharmacology
Biomedical and Life Sciences
Biomedicine
Calicophoron daubneyi
Culture Media
Health aspects
Helminthology - Original Paper
Immunology
Infection
Livestock
Medical Microbiology
Microbiology
Microscopy, Electron, Scanning
Morbidity
Oxyclozanide - pharmacology
Paramphistomatidae - drug effects
Paramphistomatidae - ultrastructure
Parasitic Sensitivity Tests - methods
Parasitic Sensitivity Tests - veterinary
Praziquantel
Praziquantel - pharmacology
Rumen
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Paramphistomosis can lead to morbidity and mortality of ruminant livestock within tropical and sub-tropical climates. In recent decades, rumen fluke has become an emerging infection in temperate climates across Western Europe, with Calicophoron daubneyi , the primary species present. Clinical outbreaks with C. daubneyi larvae are reported and adults might be responsible for production losses. There is not currently a widely licensed anthelmintic product available to control C. daubneyi. In this study, three existing flukicide anthelmintics were tested for efficacy against mature C. daubneyi , comparing a standard in vitro culturing assay and a new more relevant rumen fluid based in vitro compound screening protocol. The new rumen based screen confirmed that oxyclozanide was active against adult C. daubneyi and identified activity with praziquantel. The study highlighted the downstream value of incorporating relevant in vitro screening for anthelmintic discovery pipelines.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-021-07066-2