Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy

Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC,...

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Bibliographic Details
Published in:Biomedical optics express 2021-02, Vol.12 (2), p.836-851
Main Authors: Matthies, Levi, Gebrekidan, Medhanie T, Tegtmeyer, Jasper F, Oetter, Nicolai, Rohde, Maximilian, Vollkommer, Tobias, Smeets, Ralf, Wilczak, Waldemar, Stelzle, Florian, Gosau, Martin, Braeuer, Andreas S, Knipfer, Christian
Format: Article
Language:English
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Summary:Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC, 4300 of non-malignant lesions and 4200 of physiological mucosa were evaluated. Non-malignant lesions were distinguished from physiological mucosa with a classification accuracy of 95.3% (95.4% sensitivity, 95.2% specificity, area under the curve (AUC) 0.99). Discriminating OSCC from non-malignant lesions showed an accuracy of 88.4% (93.7% sensitivity, 76.7% specificity, AUC 0.93). OSCC was identified against physiological mucosa with an accuracy of 89.8% (93.7% sensitivity, 81.0% specificity, AUC 0.90). These findings underline the potential of SERDS for the diagnosis of oral cavity lesions.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.409456