Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission, and disease. Here, we present a two-component protein...

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Bibliographic Details
Published in:	Cell 2021-03, Vol.184 (5), p.1188-1200.e19
Main Authors:	Brouwer, Philip J.M., Brinkkemper, Mitch, Maisonnasse, Pauline, Dereuddre-Bosquet, Nathalie, Grobben, Marloes, Claireaux, Mathieu, de Gast, Marlon, Marlin, Romain, Chesnais, Virginie, Diry, Ségolène, Allen, Joel D., Watanabe, Yasunori, Giezen, Julia M., Kerster, Gius, Turner, Hannah L., van der Straten, Karlijn, van der Linden, Cynthia A., Aldon, Yoann, Naninck, Thibaut, Bontjer, Ilja, Burger, Judith A., Poniman, Meliawati, Mykytyn, Anna Z., Okba, Nisreen M.A., Schermer, Edith E., van Breemen, Marielle J., Ravichandran, Rashmi, Caniels, Tom G., van Schooten, Jelle, Kahlaoui, Nidhal, Contreras, Vanessa, Lemaître, Julien, Chapon, Catherine, Fang, Raphaël Ho Tsong, Villaudy, Julien, Sliepen, Kwinten, van der Velden, Yme U., Haagmans, Bart L., de Bree, Godelieve J., Ginoux, Eric, Ward, Andrew B., Crispin, Max, King, Neil P., van der Werf, Sylvie, van Gils, Marit J., Le Grand, Roger, Sanders, Rogier W.
Format:	Article
Language:	eng
Subjects:	Animals antibodies Antibodies, Neutralizing B cells B-Lymphocytes - immunology COVID-19 COVID-19 - immunology COVID-19 - prevention & control COVID-19 Vaccines - administration & dosage COVID-19 Vaccines - immunology immunity Life Sciences Macaca fascicularis macaques Mice Mice, Inbred BALB C Models, Animal nanoparticles Nanoparticles - administration & dosage protection Rabbits SARS-CoV-2 SARS-CoV-2 - physiology Spike Glycoprotein, Coronavirus - blood Spike Glycoprotein, Coronavirus - chemistry T-Lymphocytes - immunology vaccine Viral Load
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Summary:	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission, and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits, and cynomolgus macaques. The vaccine-induced immunity protects macaques against a high-dose challenge, resulting in strongly reduced viral infection and replication in the upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic. [Display omitted] •Two-component protein nanoparticles display multiple copies of the SARS-CoV-2 spike•Spike protein nanoparticles enhance cognate B cell activation in vitro•Vaccination induces potent neutralization in mice, rabbits, and cynomolgus macaques•Vaccination protects macaques against a high-dose SARS-CoV-2 challenge Brouwer et al. present preclinical evidence in support of a COVID-19 vaccine candidate, designed as a self-assembling two-component protein nanoparticle displaying multiple copies of the SARS-CoV-2 spike protein, which induces strong neutralizing antibody responses and protects from high-dose SARS-CoV-2 challenge.
ISSN:	0092-8674 1097-4172