Group II Metabotropic Glutamate Receptors Modulate Sound Evoked and Spontaneous Activity in the Mouse Inferior Colliculus

Little is known about the functions of Group II metabotropic glutamate receptors (mGluRs2/3) in the inferior colliculus (IC), a midbrain structure that is a major integration region of the central auditory system. We investigated how these receptors modulate sound-evoked and spontaneous firing in th...

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Bibliographic Details
Published in:eNeuro 2021-01, Vol.8 (1), p.ENEURO.0328-20.2020
Main Authors: Kristaponyte, Inga, Beebe, Nichole L, Young, Jesse W, Shanbhag, Sharad J, Schofield, Brett R, Galazyuk, Alexander V
Format: Article
Language:English
Subjects:
Animals
Inferior Colliculi
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
New Research
Receptors, Metabotropic Glutamate - genetics
Sound
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Summary:Little is known about the functions of Group II metabotropic glutamate receptors (mGluRs2/3) in the inferior colliculus (IC), a midbrain structure that is a major integration region of the central auditory system. We investigated how these receptors modulate sound-evoked and spontaneous firing in the mouse IC We first performed immunostaining and tested hearing thresholds to validate vesicular GABA transporter (VGAT)-ChR2 transgenic mice on a mixed CBA/CaJ x C57BL/6J genetic background. Transgenic animals allowed for optogenetic cell-type identification. Extracellular single neuron recordings were obtained before and after pharmacological mGluR2/3 activation. We observed increased sound-evoked firing, as assessed by the rate-level functions (RLFs), in a subset of both GABAergic and non-GABAergic IC neurons following mGluR2/3 pharmacological activation. These neurons also displayed elevated spontaneous excitability and were distributed throughout the IC area tested, suggesting a widespread mGluR2/3 distribution in the mouse IC.
ISSN:2373-2822
2373-2822
DOI:10.1523/ENEURO.0328-20.2020