A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores

The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Her...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2021-04, Vol.177, p.113005-113005, Article 113005
Main Authors: Kim, Hwang-soo, Abbas, Naseem, Shin, Sehyun
Format: Article
Language:English
Subjects:
Biosensing Techniques - economics
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
COVID-19
COVID-19 - diagnosis
COVID-19 - virology
COVID-19 Nucleic Acid Testing - economics
COVID-19 Nucleic Acid Testing - instrumentation
COVID-19 Nucleic Acid Testing - methods
DNA Probes - chemistry
DNA Probes - genetics
Equipment Design
Humans
Hydrogel
Hydrogels - chemistry
Immobilized Nucleic Acids - chemistry
Immobilized Nucleic Acids - genetics
Lab-On-A-Chip Devices
Limit of Detection
Microfluidics
Micropores
Molecular Diagnostic Techniques - economics
Molecular Diagnostic Techniques - instrumentation
Molecular Diagnostic Techniques - methods
Nucleic acid
Nucleic Acid Amplification Techniques - economics
Nucleic Acid Amplification Techniques - instrumentation
Nucleic Acid Amplification Techniques - methods
Point-of-Care Testing
Rolling circle amplification
SARS-CoV-2 - genetics
SARS-CoV-2 - isolation & purification
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Summary:The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device. •Rapid DNA gelation formed with modified RCA using a dumbbell-shaped template.•The smaller pores of nylon mesh, the quicker the blockage can occur.•Significant enhancement in detection of clogging pores with precision microfluidics.•The LOD of SARS-CoV-2 was 0.7 aM within 15 min incubation, equivalent to conventional PCR performance.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2021.113005