Nucleolin regulates the proliferation of vascular smooth muscle cells in atherosclerotic via Aurora B

Vascular smooth muscle cells (VSMCs) play a significant role in atherosclerosis. As a multifunctional protein, nucleolin (NCL) is involved in many important physiological and pathological processes. In this study, we aimed to investigate the role of nucleolin in VSMCs proliferation and cell cycle. T...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2021-01, Vol.25 (2), p.751-762
Main Authors: Sun, Hui, Huang, Lingjin, Liang, Pengfei, Tang, Yuting, Chen, Cheng, Chen, Huan, Lin, Xiaofang, Luo, Zhengyang, Li, Ying, Jiang, Bimei, Xiao, Xianzhong
Format: Article
Language:English
Subjects:
Ablation
Animals
Antibodies
Apolipoproteins E - genetics
Apolipoproteins E - metabolism
Apoptosis
Arteriosclerosis
Atherosclerosis
Atherosclerosis - genetics
Atherosclerosis - metabolism
Aurora B
Aurora B protein
Aurora Kinase B - genetics
Aurora Kinase B - metabolism
Bioinformatics
Blotting, Western
Carotid arteries
Carotid artery
Cell cycle
Cell Cycle - physiology
Cell division
Cell growth
Cell proliferation
Cell Proliferation - genetics
Cell Proliferation - physiology
Cell Survival - physiology
Cells, Cultured
Diet
DNA biosynthesis
Experiments
Gene expression
Humans
Immunofluorescence
Kinases
Lipoproteins, LDL - metabolism
Localization
Low density lipoprotein
Male
Mice
mRNA
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - metabolism
Myocytes, Smooth Muscle - metabolism
Nucleolin
Original
Phosphoproteins - genetics
Phosphoproteins - metabolism
Plaques
Protein interaction
Proteins
Reagents
RNA, Messenger - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
S phase
Smooth muscle
Surgery
Variance analysis
vascular smooth muscle cells
Veins & arteries
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Summary:Vascular smooth muscle cells (VSMCs) play a significant role in atherosclerosis. As a multifunctional protein, nucleolin (NCL) is involved in many important physiological and pathological processes. In this study, we aimed to investigate the role of nucleolin in VSMCs proliferation and cell cycle. The expression of nucleolin increased in VSMCs of mice with aortas advanced plaques. With the left common carotid‐artery ligation‐injury model, immunofluorescence staining revealed that nucleolin and Ki67 expression increased in VSMCs in mice left carotid artery compared with right carotid artery after surgery. POVPC or ox‐LDL up‐regulated nucleolin mRNA and protein expression in a dose‐ and time‐dependent manner in HAVSMCs. POVPC (5μg/ml) or ox‐LDL (50μg/ml) promoted the proliferation of HAVSMCs. Nucleolin ablation relieved the pro‐proliferation role of VSMCs. The cell cycle assay and cell ability results showing that POVPC or ox‐LDL increased the proliferation, but nucleolin ablation inhibited the proliferation of HAVSMCs. And nucleolin ablation can prevent DNA replication at S phase and induce cell cycle arrest in S phase. The bioinformatics database predicts protein‐protein interactions with nucleolin and aurora B. Nucleolin overexpression and ablation affected the expression of aurora B. These findings indicate for the first time that nucleolin actively involved the proliferation of VSMCs via aurora B.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.16125