Ethyl acetate fraction of flavonoids from Polygonum hydropiper L. modulates pseudorabies virus-induced inflammation in RAW264.7 cells via the nuclear factor-kappa B and mitogen-activated protein kinase pathways

Pseudorabies virus (PRV) infection leads to severe inflammatory responses and tissue damage, and many natural herbs exhibit protective effects against viral infection by modulating the inflammatory response. An ethyl acetate fraction of flavonoids from Polygonum hydropiper L. (FEA) was prepared thro...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2020, Vol.82(12), pp.1781-1792
Main Authors: REN, Chun-Zhi, HU, Wen-Yue, LI, Jun-Cheng, XIE, Ying-Hong, JIA, Ni-Na, SHI, Jun, WEI, Ying-Yi, HU, Ting-Jun
Format: Article
Language:English
Subjects:
Acetates
Acetic acid
Animals
Anti-inflammatory agents
Cell viability
Cyclooxygenase-2
Cytokines
Ethanol
Ethyl acetate
ethyl acetate fraction
Flavonoids
Gene expression
Herpesvirus 1, Suid - metabolism
Infections
Inflammation
Inflammation - drug therapy
Inflammation - veterinary
Kinases
Lipopolysaccharides
Macrophages - metabolism
MAP kinase
Mice
Mitogen-Activated Protein Kinases - metabolism
Natural products
NF-kappa B - metabolism
NF-κB protein
Nitric oxide
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - metabolism
Nitric-oxide synthase
Nuclear transport
Phosphorylation
Polygonum - metabolism
Polygonum hydropiper
Polygonum hydropiper L
Protein kinase
Pseudorabies
pseudorabies virus
Rabbits
Reactive oxygen species
Signal transduction
Swine
Swine Diseases
Viral infections
Virology
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Summary:Pseudorabies virus (PRV) infection leads to severe inflammatory responses and tissue damage, and many natural herbs exhibit protective effects against viral infection by modulating the inflammatory response. An ethyl acetate fraction of flavonoids from Polygonum hydropiper L. (FEA) was prepared through ethanol extraction and ethyl acetate fractional extraction. An inflammatory model was established in RAW264.7 cells with PRV infection to evaluate the anti-inflammatory activity of FEA by measuring cell viability, nitric oxide (NO) production, reactive oxygen species (ROS) release, and mRNA expression of inflammatory factors, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Its functional mechanism was investigated by analyzing the phosphorylation and nuclear translocation of key proteins in the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Our findings indicate that PRV induced inflammatory responses in RAW264.7 cells, and the responses were similar to that in lipopolysaccharide (LPS)-stimulated cells. FEA significantly suppressed NO synthesis and down-regulated both expression and secretion of COX-2, iNOS, and inflammatory cytokines (P
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.20-0263