Photobiomodulation Therapy in the Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells: An In Vitro Study

Since photobiomodulation therapy (PBMT) favors mesenchymal stem cell (MSC) preconditioning before MSC transplantation, increasing the proliferation of these cells without molecular injuries by conserving their characteristics, in the present study we analyzed the effect of PBMT on the proliferation...

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Published in:Journal of lasers in medical sciences 2020, Vol.11 (4), p.469-474
Main Authors: Miranda, Jéssica Meirinhos, de Arruda, José Alcides Almeida, Moreno, Lara Marques Magalhães, Gaião, Wyndly Daniel Cardoso, do Nascimento, Sinval Vinícius Barbosa, Silva, Eduardo Vinícius de Souza, da Silva, Márcia Bezerra, Rodrigues, Cláudio Gabriel, de Albuquerque, Diana Santana, Braz, Rodivan, Pinheiro, Antonio Luiz Barbosa, Gerbi, Marleny Elizabeth Marquez de Martinez
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Language:English
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Summary:Since photobiomodulation therapy (PBMT) favors mesenchymal stem cell (MSC) preconditioning before MSC transplantation, increasing the proliferation of these cells without molecular injuries by conserving their characteristics, in the present study we analyzed the effect of PBMT on the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs). Irradiation with an InGaAIP Laser (660 nm, 10 mW, 2.5 J/cm , 0.08 cm spot size, and 10 s) was carried out. The cells were divided into four groups: CONTROL [cells grown in Dulbecco's Modified Eagle Medium (DMEM)], OSTEO (cells grown in an osteogenic medium); PBMT (cells grown in DMEM+PBMT), and OSTEO+PBMT (cells grown in an osteogenic medium plus PBMT). The cell proliferation curve was obtained over periods of 24, 48 and 72 hours using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Osteogenic differentiation was analyzed by the formation of calcium nodules over periods of 7, 14 and 21 days. Morphometric analysis was performed to quantify the total area of nodular calcification. The highest cell proliferation and cell differentiation occurred in the OSTEO+PBMT group, followed by the PBMT, OSTEO and CONTROL groups respectively, at the observed times (
ISSN:2008-9783
2228-6721
DOI:10.34172/jlms.2020.73