Alterations in blood microbiota after colonic cancer surgery

Background Mechanisms contributing to the perioperative stress response remain poorly understood. This study investigated changes in the amount of bacterial DNA in blood and the diversity of blood microbiota in the perioperative period in patients undergoing minimally invasive surgery for colonic ca...

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Bibliographic Details
Published in:BJS open 2020-12, Vol.4 (6), p.1227-1237
Main Authors: Søby, J. H., Watt, S. K., Vogelsang, R. P., Servant, F., Lelouvier, B., Raskov, H., Knop, F. K., Gögenur, I.
Format: Article
Language:English
Subjects:
Blood cancer
Cancer surgery
Colorectal cancer
Deoxyribonucleic acid
Disease
DNA
Laparoscopy
Lower GI
Lymphatic system
Microbiota
Mortality
Nausea
Original
Plasmids
Recovery (Medical)
Skin
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Summary:Background Mechanisms contributing to the perioperative stress response remain poorly understood. This study investigated changes in the amount of bacterial DNA in blood and the diversity of blood microbiota in the perioperative period in patients undergoing minimally invasive surgery for colonic cancer in an enhanced recovery after surgery setting. Methods DNA encoding the bacterial 16S ribosomal RNA gene (16S rDNA) in whole blood obtained the day before surgery, and on postoperative day (POD) 1 and POD 10–14 was amplified and quantified by PCR before sequencing for taxonomic assignment. Richness, evenness and similarity measures were calculated to compare microbiota between days. Differences in relative abundance were analysed using the linear discriminant analysis effect size (LEfSe) algorithm. Results Thirty patients were included between January and July 2016. The concentration of bacterial 16S rDNA in blood increased between the day before surgery and POD 1 (P = 0.025). Bacterial richness was lower on POD 10–14 than on the day before surgery and POD 1 (both P 
ISSN:2474-9842
2474-9842
DOI:10.1002/bjs5.50357