Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression

Bladder cancer (BC) is one of the most commonly diagnosed urologic carcinomas, with high recurrence and death rates. Circular RNAs (circRNAs) are a class of noncoding RNAs which are anomalously expressed in cancers and involved in the progression of cancers. In this study, we found that circSEMA5A w...

Full description

Saved in:
Bibliographic Details
Published in:Aging (Albany, NY.) NY.), 2020-11, Vol.12 (21), p.21674-21686
Main Authors: Wang, Lei, Li, Haoran, Qiao, Qingdong, Ge, Yukun, Ma, Ling, Wang, Qiang
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - biosynthesis
Cell Line, Tumor
Disease Progression
DNA-Binding Proteins - biosynthesis
Gene Expression Regulation, Neoplastic - physiology
Humans
MicroRNAs - genetics
MicroRNAs - metabolism
Phosphopyruvate Hydratase - biosynthesis
Research Paper
RNA, Circular - genetics
RNA, Circular - metabolism
Semaphorins - metabolism
Tumor Cells, Cultured
Tumor Suppressor Proteins - biosynthesis
Up-Regulation
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bladder cancer (BC) is one of the most commonly diagnosed urologic carcinomas, with high recurrence and death rates. Circular RNAs (circRNAs) are a class of noncoding RNAs which are anomalously expressed in cancers and involved in the progression of cancers. In this study, we found that circSEMA5A was upregulated in BC tissues and cell lines. The overexpressed circSEMA5A was correlated with malignant characteristics of BC. In vitro data indicated that circSEMA5A promoted proliferation, suppressed apoptosis, facilitated migration, accelerated invasion, enhanced angiogenesis and promotes glycolysis of BC. Mechanistically, circSEMA5A served as a miRNA sponge for miR-330-5p to upregulates Enolase 1 (ENO1) expression and facilitated the activation of Akt and β-catenin signaling pathways. Then, we showed that circSEMA5A exerted its biological functions partially via miR-330-5p/ENO1 signaling. Moreover, circSEMA5A raised SEMA5A expression by recruiting EIF4A3 to enhance the mRNA stability of SEMA5A, and thereby accelerated BC angiogenesis. To sum up, circSEMA5A is upregulated in BC and facilitates BC progression by mediating miR-330-5p/ENO1 signaling and upregulating SEMA5A expression.
ISSN:1945-4589
1945-4589
DOI:10.18632/aging.103971