HbWRKY27, a group IIe WRKY transcription factor, positively regulates HbFPS1 expression in Hevea brasiliensis

Farnesyl pyrophosphate synthase (FPS) is a key enzyme that catalyzes the formation of farnesyl pyrophosphate, the main initiator for rubber chain initiation in Hevea brasiliensis Muell. Arg. The transcriptional regulatory mechanisms of the FPS gene still not well understood. Here, a WRKY transcripti...

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Published in:Scientific reports 2020-11, Vol.10 (1), p.20639-20639, Article 20639
Main Authors: Qu, Long, Li, Hui-Liang, Guo, Dong, Wang, Ying, Zhu, Jia-Hong, Yin, Li-Yan, Peng, Shi-Qing
Format: Article
Language:English
Subjects:
631/337
631/449
Abscisic acid
Acetates - metabolism
Amino Acid Sequence
Cell Nucleus - genetics
Cyclopentanes - metabolism
Farnesyl pyrophosphate synthase
FPS gene
Gene Expression Regulation, Plant - genetics
Genes, Plant - genetics
Hevea - genetics
Hevea - metabolism
Hevea brasiliensis
Humanities and Social Sciences
Latex
Latex - metabolism
Methyl jasmonate
multidisciplinary
Nicotiana - genetics
Nicotiana - metabolism
Oxylipins - metabolism
Plant Growth Regulators - genetics
Plant Proteins - genetics
Promoter Regions, Genetic - genetics
Rubber
Rubber - metabolism
Salicylic acid
Science
Science (multidisciplinary)
Tobacco
Transcription factors
Transcription Factors - genetics
Up-Regulation - genetics
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Summary:Farnesyl pyrophosphate synthase (FPS) is a key enzyme that catalyzes the formation of farnesyl pyrophosphate, the main initiator for rubber chain initiation in Hevea brasiliensis Muell. Arg. The transcriptional regulatory mechanisms of the FPS gene still not well understood. Here, a WRKY transcription factor designated HbWRKY27 was obtained by screening the latex cDNA library applied the HbFPS1 promoter as bait. HbWRKY27 interacted with the HbFPS1 promoter was further identified by individual Y1H and EMSA assays. HbWRKY27 belongs to group IIe WRKY subfamily which contains a typical WRKY domain and C-X5-CX23-HXH motif. HbWRKY27 was localized to the nucleus. HbWRKY27 predominantly accumulated in latex. HbWRKY27 was up-regulated in latex by ethrel, salicylic acid, abscisic acid, and methyl jasmonate treatment. Transient expression of HbWRKY27 led to increasing the activity of the HbFPS1 promoter in tobacco plant, suggesting that HbWRKY27 positively regulates the HbFPS1 expression. Taken together, an upstream transcription factor of the key natural rubber biosynthesis gene HbFPS1 was identified and this study will provide novel transcriptional regulatory mechanisms of the FPS gene in Hevea brasiliensis .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-77805-5