Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Transcription of SARS-CoV-2 mRNA requires sequential reactions facilitated by the replication and transcription complex (RTC). Here, we present a structural snapshot of SARS-CoV-2 RTC as it transitions toward cap structure synthesis. We determine the atomic cryo-EM structure of an extended RTC assem...

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Bibliographic Details
Published in:Cell 2021-01, Vol.184 (1), p.184-193.e10
Main Authors: Yan, Liming, Ge, Ji, Zheng, Litao, Zhang, Ying, Gao, Yan, Wang, Tao, Huang, Yucen, Yang, Yunxiang, Gao, Shan, Li, Mingyu, Liu, Zhenyu, Wang, Haofeng, Li, Yingjian, Chen, Yu, Guddat, Luke W., Wang, Quan, Rao, Zihe, Lou, Zhiyong
Format: Article
Language:English
Subjects:
Amino Acid Sequence
cap
Coronavirus - chemistry
Coronavirus - classification
Coronavirus - enzymology
Coronavirus RNA-Dependent RNA Polymerase - chemistry
Coronavirus RNA-Dependent RNA Polymerase - metabolism
cryo-EM
Cryoelectron Microscopy
Methyltransferases - metabolism
Models, Molecular
mRNA synthesis
nsp9
replication and transcription complex
RNA Helicases - metabolism
RNA, Messenger - chemistry
RNA, Viral - chemistry
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - metabolism
SARS-CoV-2
SARS-CoV-2 - chemistry
SARS-CoV-2 - enzymology
Sequence Alignment
Transcription, Genetic
Viral Nonstructural Proteins - chemistry
Viral Nonstructural Proteins - metabolism
Viral Replicase Complex Proteins - chemistry
Virus Replication
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Summary:Transcription of SARS-CoV-2 mRNA requires sequential reactions facilitated by the replication and transcription complex (RTC). Here, we present a structural snapshot of SARS-CoV-2 RTC as it transitions toward cap structure synthesis. We determine the atomic cryo-EM structure of an extended RTC assembled by nsp7-nsp82-nsp12-nsp132-RNA and a single RNA-binding protein, nsp9. Nsp9 binds tightly to nsp12 (RdRp) NiRAN, allowing nsp9 N terminus inserting into the catalytic center of nsp12 NiRAN, which then inhibits activity. We also show that nsp12 NiRAN possesses guanylyltransferase activity, catalyzing the formation of cap core structure (GpppA). The orientation of nsp13 that anchors the 5′ extension of template RNA shows a remarkable conformational shift, resulting in zinc finger 3 of its ZBD inserting into a minor groove of paired template-primer RNA. These results reason an intermediate state of RTC toward mRNA synthesis, pave a way to understand the RTC architecture, and provide a target for antiviral development. [Display omitted] •Structure of SARS-CoV-2 elongation complex with nsp9 has been determined•Nsp9 binds to the catalytic center of the nsp12 (RdRp) NiRAN domain•The nsp12 NiRAN domain catalyzes the formation of the cap core structure (GpppA)•The structure reveals an intermediate state for RTC in cap synthesis Yan et al. present a cryo-EM structure of the SARS-CoV-2 replication and transcription complex that includes the single RNA-binding protein nsp9. The structural snapshot provides insight into how the viral machinery catalyzes a crucial step in viral mRNA cap synthesis, which is important for virus survival.
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2020.11.016