Long-term, high-spatiotemporal resolution recording from cultured organotypic slices with high-density microelectrode arrays

A novel system to cultivate and record brain slices directly on high-density microelectrode arrays (HD-MEA) was developed. This system allows to continuously record electrical activity of selected individual neurons at high spatial resolution, while monitoring neuronal network activity at the same t...

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Bibliographic Details
Published in:2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) Actuators and Microsystems (TRANSDUCERS), 2015-06, Vol.18, p.1037-1040
Main Authors: Gong, W., Sencar, J., Jackel, D., Muller, J., Fiscella, M., Radivojevic, M., Bakkum, D., Hierlemann, A.
Format: Article
Language:English
Subjects:
Biological neural networks
Extracellular
High-density microelectrode arrays
long-term experiment
Microelectrodes
Neurons
organotypic hippocampal slice
single neuron tracing
Spatial resolution
Surface impedance
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Summary:A novel system to cultivate and record brain slices directly on high-density microelectrode arrays (HD-MEA) was developed. This system allows to continuously record electrical activity of selected individual neurons at high spatial resolution, while monitoring neuronal network activity at the same time. For the first time, properties of single neurons and the corresponding neuronal network in an organotypic hippocampal slice culture were studied over four consecutive weeks at daily intervals.
ISSN:2159-547X
2167-0013
2167-0021
DOI:10.1109/TRANSDUCERS.2015.7181103