Capillary Electrophoresis-Mass Spectrometry at Trial by Metabo-Ring: Effective Electrophoretic Mobility for Reproducible and Robust Compound Annotation

Capillary zone electrophoresis-mass spectrometry (CE-MS) is a mature analytical tool for the efficient profiling of (highly) polar and ionizable compounds. However, the use of CE-MS in comparison to other separation techniques remains underrepresented in metabolomics, as this analytical approach is...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-10, Vol.92 (20), p.14103-14112
Main Authors: Drouin, Nicolas, van Mever, Marlien, Zhang, Wei, Tobolkina, Elena, Ferre, Sabrina, Servais, Anne-Catherine, Gou, Marie-Jia, Nyssen, Laurent, Fillet, Marianne, Lageveen-Kammeijer, Guinevere S M, Nouta, Jan, Chetwynd, Andrew J, Lynch, Iseult, Thorn, James A, Meixner, Jens, Lößner, Christopher, Taverna, Myriam, Liu, Sylvie, Tran, N Thuy, Francois, Yannis, Lechner, Antony, Nehmé, Reine, Al Hamoui Dit Banni, Ghassan, Nasreddine, Rouba, Colas, Cyril, Lindner, Herbert H, Faserl, Klaus, Neusüß, Christian, Nelke, Manuel, Lämmerer, Stefan, Perrin, Catherine, Bich-Muracciole, Claudia, Barbas, Coral, Gonzálvez, Ángeles López, Guttman, Andras, Szigeti, Marton, Britz-McKibbin, Philip, Kroezen, Zachary, Shanmuganathan, Meera, Nemes, Peter, Portero, Erika P, Hankemeier, Thomas, Codesido, Santiago, González-Ruiz, Víctor, Rudaz, Serge, Ramautar, Rawi
Format: Article
Language:English
Subjects:
Analytical chemistry
Annotations
Biomarkers
Blood plasma
Capillary electrophoresis
Capillary zone
Cations
Cations - chemistry
Chemical Sciences
Chemistry
Databases, Chemical
Databases, Chemical Electrolytes
Electrolytes - chemistry
Electrophoresis
Electrophoresis, Capillary
Electrophoresis, Capillary - methods
Electrophoretic mobility
Heterogeneity
Human health sciences
Humans
Interlaboratory study
Ions
Laboratories
Laboratory tests
Mass spectrometry
Mass spectroscopy
Metabolite annotation
Metabolites
Metabolome
Metabolomics
Mobility
Organic Chemicals
Organic Chemicals - blood
Organic Chemicals - urine
Parameters
Pharmacie, pharmacologie & toxicologie
Pharmacy, pharmacology & toxicology
Platforms
Reproducibility
Reproducibility of Results
Sciences de la santé humaine
Scientific imaging
Separation techniques
Spectroscopy
Tandem Mass Spectrometry
Tandem Mass Spectrometry - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Capillary zone electrophoresis-mass spectrometry (CE-MS) is a mature analytical tool for the efficient profiling of (highly) polar and ionizable compounds. However, the use of CE-MS in comparison to other separation techniques remains underrepresented in metabolomics, as this analytical approach is still perceived as technically challenging and less reproducible, notably for migration time. The latter is key for a reliable comparison of metabolic profiles and for unknown biomarker identification that is complementary to high resolution MS/MS. In this work, we present the results of a Metabo-ring trial involving 16 CE-MS platforms among 13 different laboratories spanning two continents. The goal was to assess the reproducibility and identification capability of CE-MS by employing effective electrophoretic mobility (μ ) as the key parameter in comparison to the relative migration time (RMT) approach. For this purpose, a representative cationic metabolite mixture in water, pretreated human plasma, and urine samples spiked with the same metabolite mixture were used and distributed for analysis by all laboratories. The μ was determined for all metabolites spiked into each sample. The background electrolyte (BGE) was prepared and employed by each participating lab following the same protocol. All other parameters (capillary, interface, injection volume, voltage ramp, temperature, capillary conditioning, and rinsing procedure, etc.) were left to the discretion of the contributing laboratories. The results revealed that the reproducibility of the μ for 20 out of the 21 model compounds was below 3.1% vs 10.9% for RMT, regardless of the huge heterogeneity in experimental conditions and platforms across the 13 laboratories. Overall, this Metabo-ring trial demonstrated that CE-MS is a viable and reproducible approach for metabolomics.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.0c03129