Comparison between paramagnetic and CD71 magnetic activated cell sorting of fetal nucleated red blood cells from the maternal blood

Background Fetal nucleated red blood cells (NRBC) from maternal circulation are rare events but can be enriched and used to evaluate the genetics of the fetus. We compared two simplified selection methods of the fetal cells from the maternal blood. Methods We isolated fetal cells from maternal blood...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2020-09, Vol.34 (9), p.e23420-n/a
Main Authors: Nemescu, Dragos, Constantinescu, Daniela, Gorduza, Vlad, Carauleanu, Alexandru, Caba, Lavinia, Navolan, Dan Bogdan
Format: Article
Language:English
Subjects:
Amniocentesis
CD71
Centrifugation
Erythrocytes
Fetuses
Fluorescence in situ hybridization
fluorescent in situ hybridization
Hemoglobin
Magnetic fields
magnetic‐activated cell sorting
Methemoglobin
Monoclonal antibodies
Normal distribution
nucleated red blood cells
Peripheral blood
Pregnancy
prenatal diagnosis
Ultrasonic imaging
Y chromosomes
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Summary:Background Fetal nucleated red blood cells (NRBC) from maternal circulation are rare events but can be enriched and used to evaluate the genetics of the fetus. We compared two simplified selection methods of the fetal cells from the maternal blood. Methods We isolated fetal cells from maternal blood through double‐density gradient centrifugation followed either by magnetic cell selection, based on the paramagnetic proprieties of the NRBC hemoglobin, converted to methemoglobin, or by a positive magnetic‐activated cell sorting (MACS) enrichment, using anti‐CD71 monoclonal antibodies. Finally, the cells were identified through fluorescence in situ hybridization (FISH) with specific chromosome X and Y probes. Results We processed 10 mL of peripheral blood samples from 27 pregnant women with singleton normal male fetuses. Hemoglobin‐based enrichment isolated significantly more NRBCs: 29.7 × 104 cells than anti‐CD71 MACS: 10.1 × 104 cells (P 
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.23420