Loading…
Baseline and innate immune response characterization of a Zfp30 knockout mouse strain
Airway neutrophilia is correlated with disease severity in a number of chronic and acute pulmonary diseases, and dysregulation of neutrophil chemotaxis can lead to host tissue damage. The gene Zfp30 was previously identified as a candidate regulator of neutrophil recruitment to the lungs and secreti...
Saved in:
Published in: | Mammalian genome 2020-08, Vol.31 (7-8), p.205-214 |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Airway neutrophilia is correlated with disease severity in a number of chronic and acute pulmonary diseases, and dysregulation of neutrophil chemotaxis can lead to host tissue damage. The gene
Zfp30
was previously identified as a candidate regulator of neutrophil recruitment to the lungs and secretion of CXCL1, a potent neutrophil chemokine, in a genome-wide mapping study using the Collaborative Cross. ZFP30 is a putative transcriptional repressor with a KRAB domain capable of inducing heterochromatin formation. Using a CRISPR-mediated knockout mouse model, we investigated the role that
Zfp30
plays in recruitment of neutrophils to the lung using models of allergic airway disease and acute lung injury. We found that the
Zfp30
null allele did not affect CXCL1 secretion or neutrophil recruitment to the lungs in response to various innate immune stimuli. Intriguingly, despite the lack of neutrophil phenotype, we found there was a significant reduction in the proportion of live
Zfp30
homozygous female mutant mice produced from heterozygous matings. This deviation from the expected Mendelian ratios implicates
Zfp30
in fertility or embryonic development. Overall, our results indicate that
Zfp30
is an essential gene but does not influence neutrophilic inflammation in this particular knockout model. |
---|---|
ISSN: | 0938-8990 1432-1777 |
DOI: | 10.1007/s00335-020-09847-z |