Distinct serum metabolomic signatures of multiparous and primiparous dairy cows switched from a moderate to high-grain diet during early lactation

Introduction Feeding of high-grain diets is common in cows during early lactation, but increases the odds of metabolic derailments, which can likely be detected as undesirable shifts in the serum metabolome signature. Objectives The present study aimed to identify the metabolic signatures of the ser...

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Published in:Metabolomics 2020-09, Vol.16 (9), p.96-96, Article 96
Main Authors: Pacífico, C., Stauder, A., Reisinger, N., Schwartz-Zimmermann, H. E., Zebeli, Q.
Format: Article
Language:English
Subjects:
Amino acids
Animal lactation
Betaine
Bile acids
Biochemistry
Biomedical and Life Sciences
Biomedicine
Carboxylic acids
Carnitine
Cell Biology
Dairy cattle
Developmental Biology
Diet
Hydroxyproline
Lactation
Lecithin
Life Sciences
Lysophosphatidylcholine
Metabolism
Metabolites
Metabolomics
Molecular Medicine
Original
Original Article
Parity
Phosphatidylcholine
Proline
Taurine
Triglycerides
Valine
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Summary:Introduction Feeding of high-grain diets is common in cows during early lactation, but increases the odds of metabolic derailments, which can likely be detected as undesirable shifts in the serum metabolome signature. Objectives The present study aimed to identify the metabolic signatures of the serum metabolome of early lactation dairy cows switched from a moderate to a high-grain diet. Methods Targeted ESI-LC-MS/MS-based metabolomics was used to characterize metabolic alterations in the serum of early lactation multiparous (MP, n = 16) and primiparous (PP, n = 8) Simmental cows, according to parity and feeding phase. Data were analysed using different data mining approaches. Results Carnitine, acetylcarnitine, propionoylcarnitine, amino acid related compounds cis-4-hydroxyproline, trans-4-hydroxyproline, proline betaine, lysophosphatidylcholine PC a C16:1 and phosphatidylcholine PC ae C36:0 were identified as the key metabolites distinguishing MP from PP cows. A different serum metabolite composition during moderate and high-grain diet was also evident. Notably, cows fed high grain diet had higher serum concentrations of primary bile acids and triglycerides, but lower levels of conjugated bile acids and carboxylic acids during the first week in grain. Amino acids valine, cystine and taurine together with lysophosphatidylcholine PC a C26:0 and several phosphatidylcholines were classified as important features for cluster separation. Conclusions Our study greatly expands earlier observations on dietary effects on serum metabolome composition of cows. The altered metabolomic fingerprints clearly distinguishable by diet and cow parity hold potential to be used as early diagnostic tools for cows experiencing grain-induced metabolic disturbances.
ISSN:1573-3882
1573-3890
DOI:10.1007/s11306-020-01712-z