Identification and characterization of SET domain family genes in bread wheat (Triticum aestivum L.)

SET domain genes (SDGs) that are involved in histone methylation have been examined in many plant species, but have never been examined in bread wheat; the histone methylation caused due to SDGs is associated with regulation of gene expression at the transcription level. We identified a total of 166...

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Bibliographic Details
Published in:Scientific reports 2020-09, Vol.10 (1), p.14624-14624, Article 14624
Main Authors: Batra, Ritu, Gautam, Tinku, Pal, Sunita, Chaturvedi, Deepti, Rakhi, Jan, Irfat, Balyan, Harindra Singh, Gupta, Pushpendra Kumar
Format: Article
Language:English
Subjects:
Attraction
Automation
Dating services
Gene Expression Regulation, Plant
Genes, Plant
Genome, Plant
Plant Proteins - genetics
PR-SET Domains - genetics
Seedlings
Stress, Physiological - genetics
Triticum - genetics
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Summary:SET domain genes (SDGs) that are involved in histone methylation have been examined in many plant species, but have never been examined in bread wheat; the histone methylation caused due to SDGs is associated with regulation of gene expression at the transcription level. We identified a total of 166 bread wheat TaSDGs, which carry some interesting features including the occurrence of tandem/interspersed duplications, SSRs (simple sequence repeats), transposable elements, lncRNAs and targets for miRNAs along their lengths and transcription factor binding sites (TFBS) in the promoter regions. Only 130 TaSDGs encoded proteins with complete SET domain, the remaining 36 proteins had truncated SET domain. The TaSDG encoded proteins were classified into six classes (I-V and VII). In silico expression analysis indicated relatively higher expression (FPKM > 20) of eight of the 130 TaSDGs in different tissues, and downregulation of 30 TaSDGs under heat and drought at the seedling stage. qRT-PCR was also conducted to validate the expression of seven genes at the seedling stage in pairs of contrasting genotypes in response to abiotic stresses (water and heat) and biotic stress (leaf rust). These genes were generally downregulated in response to the three stresses examined.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-71526-5