Caveolin1 and YAP drive mechanically induced mesothelial to mesenchymal transition and fibrosis

Despite their emerging relevance to fully understand disease pathogenesis, we have as yet a poor understanding as to how biomechanical signals are integrated with specific biochemical pathways to determine cell behaviour. Mesothelial-to-mesenchymal transition (MMT) markers colocalized with TGF-β1-de...

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Bibliographic Details
Published in:Cell death & disease 2020-08, Vol.11 (8), p.647-647, Article 647
Main Authors: Strippoli, Raffaele, Sandoval, Pilar, Moreno-Vicente, Roberto, Rossi, Lucia, Battistelli, Cecilia, Terri, Michela, Pascual-Antón, Lucía, Loureiro, Marta, Matteini, Francesca, Calvo, Enrique, Jiménez-Heffernan, José Antonio, Gómez, Manuel José, Jiménez-Jiménez, Victor, Sánchez-Cabo, Fátima, Vázquez, Jesús, Tripodi, Marco, López-Cabrera, Manuel, Del Pozo, Miguel Ángel
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - metabolism
Adaptor Proteins, Signal Transducing - physiology
Animal models
Animals
Biomechanics
Caveolin
Caveolin 1 - metabolism
Caveolin 1 - physiology
Caveolin-1
Caveolins - metabolism
Disease Models, Animal
Epithelial Cells - metabolism
Epithelial-Mesenchymal Transition - genetics
Female
Fibrosis
Gene expression
Humans
Kinases
Male
Mechanotransduction
Mesenchyme
Mice
Mice, Inbred C57BL
Nuclear transport
Peritoneal Dialysis - methods
Peritoneal Fibrosis - genetics
Peritoneal Fibrosis - metabolism
Peritoneal Fibrosis - pathology
Peritoneum
Peritoneum - metabolism
Proteomes
Signal transduction
Signal Transduction - drug effects
Smad3 Protein - metabolism
Tissue Adhesions - metabolism
Transcription
Transcription Factors - metabolism
Transcription Factors - physiology
Transcriptomes
Transforming Growth Factor beta1 - metabolism
Transforming growth factor-b1
Yes-associated protein
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Summary:Despite their emerging relevance to fully understand disease pathogenesis, we have as yet a poor understanding as to how biomechanical signals are integrated with specific biochemical pathways to determine cell behaviour. Mesothelial-to-mesenchymal transition (MMT) markers colocalized with TGF-β1-dependent signaling and yes-associated protein (YAP) activation across biopsies from different pathologies exhibiting peritoneal fibrosis, supporting mechanotransduction as a central driving component of these class of fibrotic lesions and its crosstalk with specific signaling pathways. Transcriptome and proteome profiling of the response of mesothelial cells (MCs) to linear cyclic stretch revealed molecular changes compatible with bona fide MMT, which (i) overlapped with established YAP target gene subsets, and were largely dependent on endogenous TGF-β1 signaling. Importantly, TGF-β1 blockade blunts the transcriptional upregulation of these gene signatures, but not the mechanical activation and nuclear translocation of YAP per se. We studied the role therein of caveolin-1 (CAV1), a plasma membrane mechanotransducer. Exposure of CAV1-deficient MCs to cyclic stretch led to a robust upregulation of MMT-related gene programs, which was blunted upon TGF-β1 inhibition. Conversely, CAV1 depletion enhanced both TGF-β1 and TGFBRI expression, whereas its re-expression blunted mechanical stretching-induced MMT. CAV1 genetic deficiency exacerbated MMT and adhesion formation in an experimental murine model of peritoneal ischaemic buttons. Taken together, these results support that CAV1-YAP/TAZ fine-tune the fibrotic response through the modulation of MMT, onto which TGF-β1-dependent signaling coordinately converges. Our findings reveal a cooperation between biomechanical and biochemical signals in the triggering of MMT, representing a novel potential opportunity to intervene mechanically induced disorders coursing with peritoneal fibrosis, such as post-surgical adhesions.
ISSN:2041-4889
2041-4889
DOI:10.1038/s41419-020-02822-1