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Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications
Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (CaP) materials in diverse medical applications. In this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, si...
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| Published in: | Scientific reports 2020-08, Vol.10 (1), p.13738, Article 13738 |
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| description | Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (CaP) materials in diverse medical applications. In this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (
SeB-NPs). Both SeB-NPs and
SeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO
was doped at the PO
position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared
SeB-NPs had a needle-cluster-like morphology. CCK-8 analysis revealed SeB-NPs and
SeB-NPs had good cytocompatibility with osteoblasts. The antibacterial activity of the prepared
SeB-NPs was confirmed by using Gram-negative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final
SeB-NPs for biomedical applications. |
| doi_str_mv | 10.1038/s41598-020-70776-7 |
| format | article |
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SeB-NPs). Both SeB-NPs and
SeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO
was doped at the PO
position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared
SeB-NPs had a needle-cluster-like morphology. CCK-8 analysis revealed SeB-NPs and
SeB-NPs had good cytocompatibility with osteoblasts. The antibacterial activity of the prepared
SeB-NPs was confirmed by using Gram-negative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final
SeB-NPs for biomedical applications.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-70776-7</identifier><identifier>PMID: 32792661</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Calcium phosphates ; Cells, Cultured ; Cholecystokinin ; Escherichia coli - drug effects ; Fourier analysis ; Humans ; Hydroxyapatites - chemistry ; Hydroxyapatites - pharmacology ; Infrared spectroscopy ; Metal Nanoparticles - chemistry ; Microbial Sensitivity Tests - methods ; Microscopy, Electron, Transmission - methods ; Nanoparticles ; Nanostructures - chemistry ; Osteoblasts ; Particle Size ; Photoelectron spectroscopy ; Photoelectron Spectroscopy - methods ; Raman spectroscopy ; Selenium ; Selenium - chemistry ; Silver ; Silver - chemistry ; Spectrophotometry, Ultraviolet - methods ; Spectroscopy, Fourier Transform Infrared - methods ; Spectrum analysis ; Staphylococcus aureus - drug effects ; Transmission electron microscopy ; X-ray diffraction ; X-Ray Diffraction - methods</subject><ispartof>Scientific reports, 2020-08, Vol.10 (1), p.13738, Article 13738</ispartof><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-75a6587a4de14724954c4f4556f4e0e8f2ef9bccdf915c2c9c9e15e0dd20b9a33</citedby><cites>FETCH-LOGICAL-c430t-75a6587a4de14724954c4f4556f4e0e8f2ef9bccdf915c2c9c9e15e0dd20b9a33</cites><orcidid>0000-0002-6175-5883</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2433603081/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2433603081?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,38551,43930,44625,53827,53829,74769,75483</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32792661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nie, Lei</creatorcontrib><creatorcontrib>Hou, Mengjuan</creatorcontrib><creatorcontrib>Wang, Tianwen</creatorcontrib><creatorcontrib>Sun, Meng</creatorcontrib><creatorcontrib>Hou, Ruixia</creatorcontrib><title>Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (CaP) materials in diverse medical applications. In this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (
SeB-NPs). Both SeB-NPs and
SeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO
was doped at the PO
position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared
SeB-NPs had a needle-cluster-like morphology. CCK-8 analysis revealed SeB-NPs and
SeB-NPs had good cytocompatibility with osteoblasts. The antibacterial activity of the prepared
SeB-NPs was confirmed by using Gram-negative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final
SeB-NPs for biomedical applications.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Calcium phosphates</subject><subject>Cells, Cultured</subject><subject>Cholecystokinin</subject><subject>Escherichia coli - drug effects</subject><subject>Fourier analysis</subject><subject>Humans</subject><subject>Hydroxyapatites - chemistry</subject><subject>Hydroxyapatites - pharmacology</subject><subject>Infrared spectroscopy</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Microscopy, Electron, Transmission - methods</subject><subject>Nanoparticles</subject><subject>Nanostructures - chemistry</subject><subject>Osteoblasts</subject><subject>Particle Size</subject><subject>Photoelectron spectroscopy</subject><subject>Photoelectron Spectroscopy - methods</subject><subject>Raman spectroscopy</subject><subject>Selenium</subject><subject>Selenium - chemistry</subject><subject>Silver</subject><subject>Silver - 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In this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (
SeB-NPs). Both SeB-NPs and
SeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO
was doped at the PO
position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared
SeB-NPs had a needle-cluster-like morphology. CCK-8 analysis revealed SeB-NPs and
SeB-NPs had good cytocompatibility with osteoblasts. The antibacterial activity of the prepared
SeB-NPs was confirmed by using Gram-negative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final
SeB-NPs for biomedical applications.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>32792661</pmid><doi>10.1038/s41598-020-70776-7</doi><orcidid>https://orcid.org/0000-0002-6175-5883</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Nature Open Access; NCBI_PubMed Central（免费）; ProQuest - Publicly Available Content Database; Free Full-Text Journals in Chemistry; Coronavirus Research Database |
| subjects | Anti-Bacterial Agents - pharmacology Antibacterial activity Calcium phosphates Cells, Cultured Cholecystokinin Escherichia coli - drug effects Fourier analysis Humans Hydroxyapatites - chemistry Hydroxyapatites - pharmacology Infrared spectroscopy Metal Nanoparticles - chemistry Microbial Sensitivity Tests - methods Microscopy, Electron, Transmission - methods Nanoparticles Nanostructures - chemistry Osteoblasts Particle Size Photoelectron spectroscopy Photoelectron Spectroscopy - methods Raman spectroscopy Selenium Selenium - chemistry Silver Silver - chemistry Spectrophotometry, Ultraviolet - methods Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis Staphylococcus aureus - drug effects Transmission electron microscopy X-ray diffraction X-Ray Diffraction - methods |
| title | Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications |
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