Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight C-labeled disaccharide calibrants and one C-la...

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Bibliographic Details
Published in:Communications biology 2020-08, Vol.3 (1), p.425-425, Article 425
Main Authors: Wang, Zhangjie, Arnold, Katelyn, Xu, Yongmei, Pagadala, Vijayakanth, Su, Guowei, Myatt, Hannah, Linhardt, Robert J, Liu, Jian
Format: Article
Language:English
Subjects:
Biology
Disaccharides
Glycosaminoglycans
Heparan sulfate
Mass spectroscopy
Polysaccharides
Quantitative analysis
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Summary:Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight C-labeled disaccharide calibrants and one C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-020-01150-6