The histone lysine methyltransferase SETD8 regulates angiogenesis through HES-1 in human umbilical vein endothelial cells

Histone modifications, including histone lysine methylation, regulate gene expression in the vasculature, and targeting tumor blood vessels through histone modification decreases tumor growth. SETD8, a methyltransferase that catalyzes the mono-methylation of histone H4 lysine 20 is known to promote...

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Published in:Scientific reports 2020-07, Vol.10 (1), p.12089-12089, Article 12089
Main Authors: Choi, Dong Kyu, Kim, Young Kyu, Park, Sang Wook, Lee, Heejin, Lee, Seul, Kim, Sang A, Kim, Soo Jin, Lee, Junyeop, Kim, Wanil, Min, Sang-Hyun, Yu, Ji Hoon
Format: Article
Language:English
Subjects:
Actin
Actins - metabolism
Angiogenesis
Biomarkers
Blood vessels
Cell cycle
Cell Line, Tumor
Cell Proliferation
DNA methylation
Endothelial cells
Gene expression
Gene Expression Regulation
Histone H4
Histone-Lysine N-Methyltransferase - metabolism
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Lysine
Methyltransferase
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - metabolism
Neovascularization, Physiologic - genetics
Osteopontin
Pluripotency
S phase
Stem cell transplantation
Stem cells
Transcription Factor HES-1 - genetics
Tumorigenesis
Umbilical vein
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Summary:Histone modifications, including histone lysine methylation, regulate gene expression in the vasculature, and targeting tumor blood vessels through histone modification decreases tumor growth. SETD8, a methyltransferase that catalyzes the mono-methylation of histone H4 lysine 20 is known to promote tumorigenesis in various cancers and its high levels of expression are related to poor prognosis. However, the detailed mechanisms by which SETD8 stimulates tumor progression and angiogenesis are still not well understood. Recent studies have demonstrated that, in vitro, BVT-948 efficiently and selectively suppresses SETD8 activity and histone methylation levels. In this study, we showed that BVT-948-mediated SETD8 inhibition in HUVECs results in an inhibition of angiogenesis. Inhibition of SETD8 not only inhibited angiogenesis but also disrupted actin stress fiber formation and induced cell cycle arrest at S phase. These effects were accompanied by increased HES-1 expression levels, decreased osteopontin levels, and a decreased differentiation of human induced pluripotent stem cells into endothelial cells. Interestingly, BVT-948 treatment reduced pathological angiogenesis in mouse OIR model. These data illustrate the mechanisms by which SETD8 regulates angiogenesis and may enable the use of a SETD8 inhibitor to treat various pathological conditions that are known to be associated with excessive angiogenesis, including and tumor growth.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-69103-x