Curcumin Loaded in Niosomal Nanoparticles Improved the Anti-tumor Effects of Free Curcumin on Glioblastoma Stem-like Cells: an In Vitro Study

Using a novel curcumin-loaded niosome nanoparticle (CM-NP), the present study was designed to evaluate the effect of curcumin on human glioblastoma stem-like cells (GSCs). CM-NP has a diameter of ~ 60 nm and a zeta potential of ~ − 35 mV with a constant physicochemical stability. The cytotoxic effec...

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Bibliographic Details
Published in:Molecular neurobiology 2020-08, Vol.57 (8), p.3391-3411
Main Authors: Sahab-Negah, Sajad, Ariakia, Fatemeh, Jalili-Nik, Mohammad, Afshari, Amir R., Salehi, Sahar, Samini, Fariborz, Rajabzadeh, Ghadir, Gorji, Ali
Format: Article
Language:English
Subjects:
Apoptosis
Biomedical and Life Sciences
Biomedicine
Brain cancer
Brain tumors
Cell Biology
Cell cycle
Cell death
Cell proliferation
Curcumin
Cytotoxicity
Glioblastoma
Glioma
Interleukin 6
Invasiveness
Monocyte chemoattractant protein 1
mRNA
Nanoparticles
Neurobiology
Neurology
Neurosciences
NF-κB protein
Reactive oxygen species
Zeta potential
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Summary:Using a novel curcumin-loaded niosome nanoparticle (CM-NP), the present study was designed to evaluate the effect of curcumin on human glioblastoma stem-like cells (GSCs). CM-NP has a diameter of ~ 60 nm and a zeta potential of ~ − 35 mV with a constant physicochemical stability. The cytotoxic effects of free curcumin (CM) and CM-NP were investigated on GSCs obtained during the removal of a brain tumor. Both CM and CM-NP caused a dose-dependent decrease in cell proliferation and viability of GSCs. The IC50 values of CM and CM-NP on GSCs were 50 and 137 μg/ml after 24 h, respectively. CM-NP exerted significantly higher effects on GSC viability, apoptosis, cell cycle arrest, and the expression of Bax, a pro-apoptotic marker, compared with CM. In addition, the migration of GSCs was significantly impaired following the administration of CM-NP compared with CM. Furthermore, CM-NP significantly increased the values of reactive oxygen species and decreased the mRNA expressions of NF-κB and IL-6 of GSCs compared with CM. Our data also revealed that CM-NP could significantly reduce the invasiveness of GSCs compared with CM, possibly via MCP-1-mediated pathways. In addition, CM-NP exhibited a significantly greater inhibitory effect on colony formation of GSCs compared with CM. These data indicate that CM-NP exhibited stronger anti-tumor effects on GSCs than CM. Although further in vivo investigations are warranted, our results suggest that CM-NP could be an ideal carrier to deliver curcumin for potential therapeutic approaches into glioblastoma.
ISSN:0893-7648
1559-1182
DOI:10.1007/s12035-020-01922-5