Myristica lowiana Phytochemicals as Inhibitor of Plasmid Conjugation in Escherichia coli

Hexane extract and methanol fraction from the stem bark of Myristica lowiana specifically and significantly inhibited the conjugal transfer of the IncW plasmid R7K, a plasmid which harbors ampicillin-, streptomycin-, and spectinomycin-resistant genes. The transfer of this plasmid via the conjugative...

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Bibliographic Details
Published in:	Evidence-based complementary and alternative medicine 2020, Vol.2020, p.1-6
Main Authors:	Kwapong, Awo Afi, Soares, Sarah, Teo, Stephen Ping, Stapleton, Paul, Gibbons, Simon
Format:	Article
Language:	eng
Subjects:	Amino acids Ampicillin Antibiotics Antimicrobial agents Bark Caustic soda Conjugation Cytotoxicity Drug resistance E coli Escherichia coli Fatty acids Flavonoids Infrared spectroscopy Magnetic resonance spectroscopy Mass spectrometry Mass spectroscopy Methanol Methicillin NMR Nuclear magnetic resonance Nuclear magnetic resonance spectroscopy Pharmacy Phytochemicals Plasmids Sodium Spectinomycin Staphylococcus infections Sterols Streptococcus infections Streptomycin Tetracycline Tetracyclines
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Summary:	Hexane extract and methanol fraction from the stem bark of Myristica lowiana specifically and significantly inhibited the conjugal transfer of the IncW plasmid R7K, a plasmid which harbors ampicillin-, streptomycin-, and spectinomycin-resistant genes. The transfer of this plasmid via the conjugative pilli of Escherichia coli was reduced by 76.5 ± 2.0% and 79.0 ± 1.2% by hexane extract and methanol fraction of M. lowiana, respectively. The hexane extract exhibited significant anti-conjugant activity at a non-cytotoxic concentration of 100 mg/L as assessed against adult human dermal fibroblast cells. The hexane extract and methanol fraction were screened using phytochemical tests, NMR spectroscopy, IR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HRESIMS) and were found to contain terpenoids, sterols, and fatty acids.
ISSN:	1741-427X 1741-4288