Epidemiological Study on Prevalence, Serovar Diversity, Multidrug Resistance, and CTX-M-Type Extended-Spectrum β-Lactamases of Salmonella spp. from Patients with Diarrhea, Food of Animal Origin, and Pets in Several Provinces of China

A total of 2,283 isolates were recovered from 18,334 samples, including samples from patients with diarrhea, food of animal origin, and pets, across 5 provinces of China. The highest prevalence of spp. was detected in chicken meats (39.3%, 486/1,237). Fifteen serogroups and 66 serovars were identifi...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2020-06, Vol.64 (7)
Main Authors: Wang, Wei, Zhao, Li, Hu, Yujie, Dottorini, Tania, Fanning, Séamus, Xu, Jin, Li, Fengqin
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
beta-Lactamases - genetics
China - epidemiology
Diarrhea - epidemiology
Drug Resistance, Multiple
Epidemiology and Surveillance
Humans
Prevalence
Salmonella - genetics
Serogroup
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Summary:A total of 2,283 isolates were recovered from 18,334 samples, including samples from patients with diarrhea, food of animal origin, and pets, across 5 provinces of China. The highest prevalence of spp. was detected in chicken meats (39.3%, 486/1,237). Fifteen serogroups and 66 serovars were identified, with serovars Typhimurium and Enteritidis being the most dominant. Most (85.5%, 1,952/2,283) isolates exhibited resistance to ≥1 antimicrobial, and 56.4% were multidrug resistant (MDR). A total of 222 isolates harbored extended-spectrum β-lactamases (ESBLs), and 200 of these were of the CTX-M type and were mostly detected in isolates from chicken meat and turtle fecal samples. Overall, eight genes were identified, with , , , , and being the most prevalent. In total, 166 of the 222 ESBL-producing isolates had amino acid substitutions in GyrA (S83Y, S83F, D87G, D87N, and D87Y) and ParC (S80I), while the plasmid-mediated quinolone resistance (PMQR)-encoding genes , , , , and were detected in almost all isolates. Of the 15 sequence types (STs) identified in the 222 ESBLs, ST17, ST11, ST34, and ST26 ranked among the top 5 in number of isolates. Our study revealed considerable serovar diversity and a high prevalence of the co-occurrence of MDR determinants, including CTX-M-type ESBLs, quinolone resistance-determining region (QRDR) mutations, and PMQR genes. This is the first report of CTX-M-130 spp. from patients with diarrhea and QRDR mutations from turtle fecal samples. Our study emphasizes the importance of actions, both in health care settings and in the veterinary medicine sector, to control the dissemination of MDR, especially the CTX-M-type ESBL-harboring isolates.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00092-20