Rice F-bZIP transcription factors regulate the zinc deficiency response

Zinc deficiency in soils and crops is a global problem. We combine functional and phylogenetic analyses to unravel the role of rice F-bZIP homologs in the zinc deficiency response. Abstract The F-bZIP transcription factors bZIP19 and bZIP23 are the central regulators of the zinc deficiency response...

Full description

Saved in:
Bibliographic Details
Published in:Journal of experimental botany 2020-06, Vol.71 (12), p.3664-3677
Main Authors: Lilay, Grmay H, Castro, Pedro Humberto, Guedes, Joana G, Almeida, Diego M, Campilho, Ana, Azevedo, Herlander, Aarts, Mark G M, Saibo, Nelson J M, Assunção, Ana G L
Format: Article
Language:English
Subjects:
Basic-Leucine Zipper Transcription Factors - metabolism
Gene Expression Regulation, Plant
Oryza - genetics
Oryza - metabolism
Phylogeny
Plant Proteins - genetics
Plant Proteins - metabolism
Research Papers
Zinc - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Zinc deficiency in soils and crops is a global problem. We combine functional and phylogenetic analyses to unravel the role of rice F-bZIP homologs in the zinc deficiency response. Abstract The F-bZIP transcription factors bZIP19 and bZIP23 are the central regulators of the zinc deficiency response in Arabidopsis, and phylogenetic analysis of F-bZIP homologs across land plants indicates that the regulatory mechanism of the zinc deficiency response may be conserved. Here, we identified the rice F-bZIP homologs and investigated their function. OsbZIP48 and OsbZIP50, but not OsbZIP49, complement the zinc deficiency-hypersensitive Arabidopsis bzip19bzip23 double mutant. Ectopic expression of OsbZIP50 in Arabidopsis significantly increases plant zinc accumulation under control zinc supply, suggesting an altered Zn sensing in OsbZIP50. In addition, we performed a phylogenetic analysis of F-bZIP homologs from representative monocot species that supports the branching of plant F-bZIPs into Group 1 and Group 2. Our results suggest that regulation of the zinc deficiency response in rice is conserved, with OsbZIP48 being a functional homolog of AtbZIP19 and AtbZIP23. A better understanding of the mechanisms behind the Zn deficiency response in rice and other important crops will contribute to develop plant-based strategies to address the problems of Zn deficiency in soils, crops, and cereal-based human diets.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/eraa115