hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simpl...

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Bibliographic Details
Published in:	Scientific reports 2020-06, Vol.10 (1), p.9950-9950, Article 9950
Main Authors:	Hildebrand, Sven, Schueth, Anna, Wangenheim, Klaus von, Mattheyer, Christian, Pampaloni, Francesco, Bratzke, Hansjürgen, Roebroeck, Alard F, Galuske, Ralf A W
Format:	Article
Language:	eng
Subjects:	Affinity Labels - chemistry Animals Brain Brain - anatomy & histology Brain - cytology Carbocyanines - chemistry Fluorescent Dyes - chemistry Formaldehyde - chemistry Humans Lipids Lipids - chemistry Lipophilic Lysine Lysine - chemistry Mice Perchloric acid Periodic Acid - chemistry Swine Tissue Fixation - methods
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Summary:	Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
ISSN:	2045-2322 2045-2322