Short ragweed pollen promotes M2 macrophage polarization via TSLP/TSLPR/OX40L signaling in allergic inflammation

This study was to explore the role and mechanism of macrophages in pollen-triggered allergic inflammation. A murine model of short ragweed (SRW) pollen-induced experimental allergic conjunctivitis (EAC), and bone marrow (BM)-macrophages cultures were used. Typical allergic manifestations and TSLP-st...

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Bibliographic Details
Published in:Mucosal immunology 2019-09, Vol.12 (5), p.1141-1149
Main Authors: Deng, Ruzhi, Chen, Xin, Zhang, Yun, Bian, Fang, Gao, Ning, Hu, Jiaoyue, Wang, Changjun, de Souza, Rodrigo G, Lu, Fan, Pflugfelder, Stephen C, Li, De-Quan
Format: Article
Language:English
Subjects:
Animal models
Animals
Antigens, Plant - immunology
Biomarkers
Bone marrow
Conjunctiva
Conjunctivitis
Cytokines - metabolism
Disease Models, Animal
Flow cytometry
Hay fever
Hypersensitivity
Hypersensitivity - immunology
Hypersensitivity - metabolism
Immunoglobulins - metabolism
Inflammation
Interleukin 13
Interleukin 4
Interleukin 5
Lymph nodes
Lymphocytes T
Macrophage Activation - immunology
Macrophages
Macrophages - immunology
Macrophages - metabolism
Mice
Mice, Knockout
OX40 Ligand - metabolism
Ox40L protein
Phenotype
Phenotypes
Plant Extracts - immunology
Polarization
Pollen
Receptors, Cytokine - metabolism
Signal Transduction
Th2 Cells - immunology
Th2 Cells - metabolism
Thymic Stromal Lymphopoietin
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Summary:This study was to explore the role and mechanism of macrophages in pollen-triggered allergic inflammation. A murine model of short ragweed (SRW) pollen-induced experimental allergic conjunctivitis (EAC), and bone marrow (BM)-macrophages cultures were used. Typical allergic manifestations and TSLP-stimulated Th2 hyperresponse were observed in ocular surface of EAC model in wild-type (WT) mice induced by SRW. The M2 phenotype markers, Arg1, Ym1 and FIZZ1, were highly expressed by conjunctiva and draining cervical lymph nodes (CLNs) of WT-EAC mice when compared with controls, as evaluated by RT-qPCR and Immunofluorescent double staining with macrophage marker F4/80. The stimulated expression of TSLPR and OX40L by macrophage was detected in conjunctiva and CLNs by RT-qPCR, double staining, and flow cytometry. M2 macrophages were found to produce TARC and MDC. In contrast, EAC model with TSLPR mice did not show allergic signs and any increase of Th2 cytokines (IL-4, IL-5 and IL-13) and M2 markers. In vitro cultures confirmed that SRW extract stimulates expression of TSLPR, OX40L, TARC, MDC, and three M2 markers by BM-macrophages from WT mice, but not from TSLPR mice. These findings demonstrate that SRW pollen primes macrophage polarization toward to M2 phenotype via TSLP/TSLPR/OX40L signaling to amplify allergic inflammation.
ISSN:1933-0219
1935-3456
DOI:10.1038/s41385-019-0187-8