Development and selection of low-level multi-drug resistance over an extended range of sub-inhibitory ciprofloxacin concentrations in Escherichia coli

To better combat bacterial antibiotic resistance, a growing global health threat, it is imperative to understand its drivers and underlying biological mechanisms. One potential driver of antibiotic resistance is exposure to sub-inhibitory concentrations of antibiotics. This occurs in both the enviro...

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Bibliographic Details
Published in:Scientific reports 2020-05, Vol.10 (1), p.8754, Article 8754
Main Authors: Ching, Carly, Zaman, Muhammad H
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Base Sequence
Ciprofloxacin
Ciprofloxacin - administration & dosage
Ciprofloxacin - pharmacology
Contamination
DNA, Bacterial - genetics
Dose-Response Relationship, Drug
Drug resistance
Drug Resistance, Multiple, Bacterial - genetics
E coli
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Microbial Sensitivity Tests
Multidrug resistance
Phenotypes
Selection, Genetic
Time Factors
Whole Genome Sequencing
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Summary:To better combat bacterial antibiotic resistance, a growing global health threat, it is imperative to understand its drivers and underlying biological mechanisms. One potential driver of antibiotic resistance is exposure to sub-inhibitory concentrations of antibiotics. This occurs in both the environment and clinic, from agricultural contamination to incorrect dosing and usage of poor-quality medicines. To better understand this driver, we tested the effect of a broad range of ciprofloxacin concentrations on antibiotic resistance development in Escherichia coli. We observed the emergence of stable, low-level multi-drug resistance that was both time and concentration dependent. Furthermore, we identified a spectrum of single mutations in strains with resistant phenotypes, both previously described and novel. Low-level class-wide resistance, which often goes undetected in the clinic, may allow for bacterial survival and establishment of a reservoir for outbreaks of high-level antibiotic resistant infections.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-65602-z