Development and characterization of 20 polymorphic microsatellite markers for the white-bellied pangolin Phataginus tricuspis (Mammalia, Pholidota)

Pangolins, or scaly anteaters, have recently been flagshiped as one of the most illegally traded mammals, and as a corollary, as potential intermediate hosts at the origin of the COVID-19 pandemic. In order to improve the traceability of their trade, we developed 20 polymorphic microsatellite loci f...

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Bibliographic Details
Published in:Molecular biology reports 2020-06, Vol.47 (6), p.4827-4833
Main Authors: Aguillon, Samantha, Din Dipita, Alain, Lecompte, Emilie, Missoup, Alain Didier, Tindo, Maurice, Gaubert, Philippe
Format: Article
Language:English
Subjects:
Alleles
Animal Anatomy
Animal Biochemistry
Animals
Betacoronavirus - pathogenicity
Biomedical and Life Sciences
Cameroon - epidemiology
Coronavirus Infections - epidemiology
Coronavirus Infections - prevention & control
Coronavirus Infections - transmission
Coronavirus Infections - virology
COVID-19
Disease Reservoirs - virology
Eutheria - genetics
Eutheria - virology
Female
Genetic diversity
Genetic Loci
Genetic Markers
Genotype
Genotypes
Histology
Humans
Life Sciences
Linkage Disequilibrium
Male
Microsatellite Repeats
Microsatellites
Morphology
Pandemics
Pandemics - prevention & control
Phataginus tricuspis
Pholidota
Pneumonia, Viral - epidemiology
Pneumonia, Viral - prevention & control
Pneumonia, Viral - transmission
Pneumonia, Viral - virology
SARS-CoV-2
Short Communication
Zoonoses - epidemiology
Zoonoses - prevention & control
Zoonoses - transmission
Zoonoses - virology
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Summary:Pangolins, or scaly anteaters, have recently been flagshiped as one of the most illegally traded mammals, and as a corollary, as potential intermediate hosts at the origin of the COVID-19 pandemic. In order to improve the traceability of their trade, we developed 20 polymorphic microsatellite loci for the white-bellied pangolin ( Phataginus tricuspis ), the species most frequently found on African bushmeat markets. We genotyped 24 white-bellied pangolins from the Douala market, Cameroon, originating from the Ebo forest c. 75 km north-east of Douala. The number of alleles per locus ranged from 4 to 12 (mean = 6.95), and mean observed and expected heterozygosities were 0.592 (0.208–0.875) and 0.671 (0.469–0.836), respectively. Genetic diversity was higher than that cross-estimated from microsatellite loci developed for other species of pangolins. Two loci deviated from Hardy–Weinberg equilibrium and two loci showed linkage disequilibrium. Genetic variance (PCoA) was increased with the addition of 13 pangolins of unknown origin, possibly suggesting that the Douala market is fed from differentiated source populations of white-bellied pangolins. Each of the 37 individuals had a unique multilocus genotype. The unbiased probability of identity (uPI) and the probability of identity among siblings (PIsibs) were both very low (uPI = 8.443 e−21; PIsibs = 1.011 e−07). Only five microsatellite loci were needed to reach the conservative value of PIsibs 
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-020-05511-6