Oxytocin and bone quality in the femoral neck of rats in periestropause

The objective of this study is to identify whether oxytocin (OT) contributes to the reduction of osteopenia in the femoral neck of rats in periestropause. Animals in irregular estrous cycles received two NaCl injections (0.15 mol/L) or OT (134 μg/kg) over a 12-h interval, and after thirty-five days...

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Published in:Scientific reports 2020-05, Vol.10 (1), p.7937-7937, Article 7937
Main Authors: Fernandes, Fernanda, Stringhetta-Garcia, Camila Tami, Peres-Ueno, Melise Jacon, Fernandes, Fabiana, Nicola, Angela Cristina de, Castoldi, Robson Chacon, Ozaki, Guilherme, Louzada, Mário Jefferson Quirino, Chaves-Neto, Antonio Hernandes, Ervolino, Edilson, Dornelles, Rita Cássia Menegati
Format: Article
Language:English
Subjects:
Acid phosphatase (tartrate-resistant)
Alkaline phosphatase
Animals
Biomarkers
Bone Density
Bone Diseases, Metabolic - etiology
Bone Diseases, Metabolic - metabolism
Bone Diseases, Metabolic - pathology
Bone mineral density
Bone morphogenetic protein 2
Cortical bone
Disease Models, Animal
Enzymatic activity
Estrus
Female
Femur
Femur Neck - diagnostic imaging
Femur Neck - metabolism
Femur Neck - pathology
Humans
Immunohistochemistry
Mechanical properties
Osteopenia
Osteoporosis
Osteoporosis, Postmenopausal - diagnostic imaging
Osteoporosis, Postmenopausal - etiology
Osteoporosis, Postmenopausal - metabolism
Osteoporosis, Postmenopausal - pathology
Oxytocin
Oxytocin - metabolism
Phosphatase
Proline
Rats
Sodium chloride
Spectrum Analysis, Raman
X-Ray Microtomography
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Summary:The objective of this study is to identify whether oxytocin (OT) contributes to the reduction of osteopenia in the femoral neck of rats in periestropause. Animals in irregular estrous cycles received two NaCl injections (0.15 mol/L) or OT (134 μg/kg) over a 12-h interval, and after thirty-five days without treatments, the biological sample collection was performed. The oxytocin group (Ot) demonstrated the highest enzymatic activity of alkaline phosphatase (p = 0.0138), lowest enzymatic activity of tartrate-resistant acid phosphatase (p = 0.0045), higher percentage of compact bone (p = 0.0359), cortical expression of runt-related transcription factor 2 (p = 0.0101), osterix (p = 0.0101), bone morphogenetic protein-2/4 (p = 0.0101) and periostin (p = 0.0455). Furthermore, the mineral-to-matrix ratio (ν PO /Proline) was higher and type-B carbonate substitution (CO /ν PO ) was lower (p = 0.0008 and 0.0303) in Ot group. The Ot showed higher areal bone mineral density (p = 0.0050), cortical bone area (p = 0.0416), polar moment of inertia, maximum, minimum (p = 0.0480, 0.0480, 0.0035), bone volume fraction (p = 0.0166), connectivity density (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-64683-0