RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib: A randomized clinical trialy

This study aimed to evaluate the gene expression of cyclooxygenases (COXs) in an oral model of preemptive analgesia. Gingival tissue was collected during extraction of lower third molars from a randomized, triple-blind, split-mouth and placebo-controlled study. The eligible patients were randomly so...

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Published in:Journal of clinical and experimental dentistry 2020-04, Vol.12 (4), p.e371-e380
Main Authors: Medeiros-Albuquerque, Assis-Filipe, Roriz-Fonteles, Cristiane-Sá, do Nascimento-Costa, José-Jackson, Viana-Silva, José-Roberto, de Barros-Silva, Paulo-Goberlânio, Studart-Soares, Eduardo-Costa, Chaves, Filipe-Nobre, Alves-Pereira, Karuza-Maria, Ribeiro, Thyciana-Rodrigues, Gurgel-Costa, Fábio-Wildson
Format: Article
Language:English
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Summary:This study aimed to evaluate the gene expression of cyclooxygenases (COXs) in an oral model of preemptive analgesia. Gingival tissue was collected during extraction of lower third molars from a randomized, triple-blind, split-mouth and placebo-controlled study. The eligible patients were randomly sorted to receive a single dose either of ibuprofen 400mg, or etoricoxib 120 mg or a placebo, one hour prior to surgery. The temporal course of RNAm was evaluated for COX-1 and -2 by means of a quantitative polymerase chain reaction in real time (RT-qPCR) at time zero and 30 minutes after the surgical procedure began, and it was correlated with clinical parameters (pain and maximum mouth opening). There was a significant increase in COX-1 expression between T0 and T30 in ibuprofen ( =0.004) and etoricoxib ( =0.010) groups. As regards COX-2, there were increases from T0 to T30 in all groups (placebo, =0.012; ibuprofen,
ISSN:1989-5488
1989-5488
DOI:10.4317/JCED.56447