Cell lines from the Egyptian fruit bat are permissive for modified vaccinia Ankara

Bats are reservoir hosts for a spectrum of infectious diseases. Some pathogens (such as Hendra, Nipah and Marburg viruses) appear to use mainly fruit bats as reservoir. We describe designed immortalization of primary fetal cells from the Egyptian fruit bat ( Rousettus aegyptiacus) to facilitate isol...

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Bibliographic Details
Published in:Virus research 2009-10, Vol.145 (1), p.54-62
Main Authors: Jordan, Ingo, Horn, Deborah, Oehmke, Stefanie, Leendertz, Fabian H., Sandig, Volker
Format: Article
Language:English
Subjects:
AGE1.R05T
AGE1.R06E
Animals
Cell Line
Chiroptera
Chlorocebus aethiops
Cricetinae
Ducks
Female
Fetus - cytology
Fruit bat
Genetic Vectors
Green Fluorescent Proteins
Humans
Marburg virus
MVA
Permanent cell line
Poxvirus
Rousettus aegyptiacus
Vaccinia - virology
Vaccinia virus - physiology
Vero Cells
Virus Replication
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Summary:Bats are reservoir hosts for a spectrum of infectious diseases. Some pathogens (such as Hendra, Nipah and Marburg viruses) appear to use mainly fruit bats as reservoir. We describe designed immortalization of primary fetal cells from the Egyptian fruit bat ( Rousettus aegyptiacus) to facilitate isolation and characterization of pathogens associated with these mammals. Three cell lines with different properties were recovered and successful immortalization was confirmed by continuous cultivation for over 18 months. Surprisingly, the cell lines are fully permissive for a highly attenuated poxvirus, modified vaccinia Ankara (MVA). MVA is a safe and well characterized vaccine vector that cannot replicate in most mammalian cells. High permissivity of Rousettus cell lines could justify testing bats for susceptibility to MVA as a replication competent vector with low zoonotic potential to induce herd immunity in bat colonies against viruses causing rabies or haemorrhagic fevers.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2009.06.007