Ginsenoside Rg1 Performs Anti-Aging Functions by Suppressing Mitochondrial Pathway-Mediated Apoptosis and Activating Sirtuin 3 (SIRT3)/Superoxide Dismutase 2 (SOD2) Pathway in Sca-1⁺ HSC/HPC Cells of an Aging Rat Model

BACKGROUND Aging is characterized by progressive deterioration in metabolic and physiological process. The present research assessed the antagonistic effects and mechanisms of Ginsenoside Rg1 (Rg1) on aging of HSCs/HPCs. MATERIAL AND METHODS Fifty male Sprague-Dawley (SD) rats were treated and divid...

Full description

Saved in:
Bibliographic Details
Published in:Medical science monitor 2020-04, Vol.26, p.e920666-e920666
Main Authors: Zhou, Yue, Wang, Yan-Ping, He, Ying-Hong, Ding, Ji-Chao
Format: Article
Language:English
Subjects:
Aging - drug effects
Aging - metabolism
Animal Study
Animals
Apoptosis - drug effects
beta-Galactosidase - metabolism
Cellular Senescence - drug effects
Disease Models, Animal
Ginsenosides - metabolism
Ginsenosides - pharmacology
Male
Mitochondria - drug effects
Oxidation-Reduction
Rats
Rats, Sprague-Dawley
Signal Transduction
Sirtuins - metabolism
Superoxide Dismutase - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:BACKGROUND Aging is characterized by progressive deterioration in metabolic and physiological process. The present research assessed the antagonistic effects and mechanisms of Ginsenoside Rg1 (Rg1) on aging of HSCs/HPCs. MATERIAL AND METHODS Fifty male Sprague-Dawley (SD) rats were treated and divided into the following groups: Control (n=10), Model (n=10, treated with D-galactose, as aging model), Rg1 Control (n=10), Rg1 treatment (n=10), and Rg1 prevention (n=10). An aging rat model was established by subcutaneous injection with D-gal. HSC/HPC cells were stained using SA-ß-Gal staining. HSC/HPC cells were examined using flow cytometry assay. CFU-mix assay, with a few modifications, was performed. Cleaved caspase-3, B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X protein (Bax) were examined using qRT-PCR. Sirtuin 3 (SIRT3) and superoxide dismutase 2 (SOD2) expression was determined using Western blot assay and qRT-PCR. RESULTS Rg1 (treatment and prevention group) significantly decreased SA-ß-Gal-positive staining in Sca-1⁺ HSC/HPC cells compared to that of the D-gal model (p
ISSN:1643-3750
1234-1010
1643-3750
DOI:10.12659/MSM.920666