Automated radiosynthesis of [11C]UCB‐J for imaging synaptic density by positron emission tomography

An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [11C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabell...

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Bibliographic Details
Published in:Journal of labelled compounds & radiopharmaceuticals 2020-03, Vol.63 (3), p.151-158
Main Authors: Milicevic Sephton, Selena, Miklovicz, Tunde, Russell, Joseph J., Doke, Aniruddha, Li, Lei, Boros, Istvan, Aigbirhio, Franklin I.
Format: Article
Language:English
Subjects:
[11C]UCB‐J
Automation
Biomarkers
carbon‐11 radiosynthesis
Cross coupling
Cyclic GMP
Density
Glycoproteins
Palladium
Positron emission
Positron emission tomography
Protocols and Methods
Quality control
Radioactive tracers
Radiochemical analysis
Radiolabelling
Suzuki cross‐coupling
SV2A protein
Synaptic density
Synthra RNPlus
Tomography
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Summary:An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [11C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabelling precursor, and the desired radiolabelled product was isolated in 11 ± 2% (n = 7) nondecay corrected radiochemical yield and formulated as a 10% EtOH solution in saline with molar activities of 20 to 100 GBq/μmol. The method was based upon the palladium(0)‐mediated Suzuki cross‐coupling reaction and [11C]CH3I as a radiolabelling synthon. The isolated product was cGMP compliant as demonstrated by the results of quality control analysis.
ISSN:0362-4803
1099-1344
DOI:10.1002/jlcr.3828