Equine Arteritis Virus Subgenomic RNA Transcription: UV Inactivation and Translation Inhibition Studies

The expression of the genetic information of equine arteritis virus (EAV), an arterivirus, involves the synthesis of six subgenomic (sg) mRNAs. These are 5′ and 3′ coterminal since they are composed of a leader and a body sequence, which are identical to the 5′ and 3′ ends of the genome, respectivel...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1995-11, Vol.213 (2), p.364-372
Main Authors: DEN BOON, JOHAN A., SPAAN, WILLY J.M., SNIJDER, ERIC J.
Format: Article
Language:English
Subjects:
Animals
ARN MENSAJERO
ARN MESSAGER
CARTOGRAFIA
CARTOGRAPHIE
Cell Line
Cycloheximide - pharmacology
Equartevirus - genetics
Equartevirus - physiology
Equartevirus - radiation effects
Genome, Viral
INFECCION
INFECTION
Mice
Murine hepatitis virus - genetics
Murine hepatitis virus - radiation effects
PESTIVIRUS
Protein Biosynthesis
Protein Synthesis Inhibitors - pharmacology
RADIACION ULTRAVIOLETA
RAYONNEMENT ULTRAVIOLET
RNA Precursors - metabolism
RNA Replicase - biosynthesis
RNA, Messenger - biosynthesis
RNA, Viral - biosynthesis
RNA, Viral - genetics
RNA, Viral - radiation effects
Sindbis Virus - genetics
Sindbis Virus - radiation effects
SINTESIS DE PROTEINAS
SYNTHESE PROTEIQUE
Transcription, Genetic
Ultraviolet Rays
Viral Proteins - biosynthesis
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Summary:The expression of the genetic information of equine arteritis virus (EAV), an arterivirus, involves the synthesis of six subgenomic (sg) mRNAs. These are 5′ and 3′ coterminal since they are composed of a leader and a body sequence, which are identical to the 5′ and 3′ ends of the genome, respectively. Previously, it has been suggested thatcis-splicing of a genome-length precursor RNA is involved in their synthesis. This was reevaluated in a comparative analysis of the sg RNA synthesis of EAV, the coronavirus mouse hepatitis virus (MHV), and the alphavirus Sindbis virus. UV transcription mapping showed that the majority of the EAV sg RNAs made at later stages of infection is not derived from a genome-length precursor. However, complete independence of sg RNA synthesis from that of genomic RNA was never observed during the course of infection. The possibility that this resulted from UV irradiation-induced effects on the synthesis of the viral replicase was investigated by inhibiting translation using cycloheximide. For EAV, ongoing protein synthesis was found to be more important for the synthesis of sg RNA than for that of genomic RNA. In general, MHV transcription was extremely sensitive to translation inhibition, whereas EAV genomic RNA synthesis became independent ofde novoprotein synthesis late in infection.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1995.0009