Melt Electrospinning Writing of Poly‐Hydroxymethylglycolide‐co‐ε‐Caprolactone‐Based Scaffolds for Cardiac Tissue Engineering

Current limitations in cardiac tissue engineering revolve around the inability to fully recapitulate the structural organization and mechanical environment of native cardiac tissue. This study aims at developing organized ultrafine fiber scaffolds with improved biocompatibility and architecture in c...

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Bibliographic Details
Published in:Advanced healthcare materials 2017-09, Vol.6 (18), p.n/a
Main Authors: Castilho, Miguel, Feyen, Dries, Flandes‐Iparraguirre, María, Hochleitner, Gernot, Groll, Jürgen, Doevendans, Pieter A. F., Vermonden, Tina, Ito, Keita, Sluijter, Joost P. G., Malda, Jos
Format: Article
Language:English
Subjects:
Biocompatibility
Biocompatible Materials - chemistry
Caproates - chemistry
cardiac tissue engineering
cell orientation
Cells (biology)
Electrospinning
functional scaffolds
Heart
Heart - physiology
Heart diseases
Lactones - chemistry
melt electrospinning writing
Microscopy, Electron, Scanning - methods
Polyesters - chemistry
polymer processing
Polymers - chemistry
Porosity
Scaffolds
Stem cells
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Current limitations in cardiac tissue engineering revolve around the inability to fully recapitulate the structural organization and mechanical environment of native cardiac tissue. This study aims at developing organized ultrafine fiber scaffolds with improved biocompatibility and architecture in comparison to the traditional fiber scaffolds obtained by solution electrospinning. This is achieved by combining the additive manufacturing of a hydroxyl‐functionalized polyester, (poly(hydroxymethylglycolide‐co‐ε‐caprolactone) (pHMGCL), with melt electrospinning writing (MEW). The use of pHMGCL with MEW vastly improves the cellular response to the mechanical anisotropy. Cardiac progenitor cells (CPCs) are able to align more efficiently along the preferential direction of the melt electrospun pHMGCL fiber scaffolds in comparison to electrospun poly(ε‐caprolactone)‐based scaffolds. Overall, this study describes for the first time that highly ordered microfiber (4.0–7.0 µm) scaffolds based on pHMGCL can be reproducibly generated with MEW and that these scaffolds can support and guide the growth of CPCs and thereby potentially enhance their therapeutic potential. Fabrication of highly ordered microfiber scaffolds for cardiac tissue engineering is achieved by melt electrospinning writing of a hydroxyl‐functionalized polyester. These scaffolds can support and guide the growth of cardiac progenitor cells while recapitulating the mechanical environment of the native cardiac tissue. This approach provides a framework for the development of therapeutically viable in vitro engineered cardiac tissues.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201700311