Gene-centric metagenomics analysis of feline intestinal microbiome using 454 junior pyrosequencing

The feline gastrointestinal microbiota have direct influence on feline health and also human health as a reservoir for potential zoonotic pathogens and antibiotic resistant bacterial strains. In order to describe the feline gastrointestinal microbial diversity, fecal samples from cats have been char...

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Bibliographic Details
Published in:Journal of microbiological methods 2012-03, Vol.88 (3), p.369-376
Main Authors: Tun, Hein Min, Brar, Manreetpal Singh, Khin, Nina, Jun, Li, Hui, Raymond Kin-Hi, Dowd, Scot E., Leung, Frederick Chi-Ching
Format: Article
Language:English
Subjects:
Animals
antibiotic resistance
Archaea
Bacteroides
Biological and medical sciences
Cats
chickens
Cluster Analysis
descriptive studies
dogs
feces
Feces - microbiology
Feline intestinal microbiome
Firmicutes
Fundamental and applied biological sciences. Psychology
fungi
gastrointestinal system
Gastrointestinal Tract - microbiology
genes
hosts
human health
humans
intestinal microorganisms
Metabolic Networks and Pathways - genetics
Metagenome
Metagenomics
Metagenomics - methods
mice
Microbiology
microbiome
nutritional intervention
pathogens
Phylogeny
Proteobacteria
Pyrosequencing
sequence analysis
Sequence Analysis, DNA - methods
titanium
viruses
Zoonotic pathogens
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Summary:The feline gastrointestinal microbiota have direct influence on feline health and also human health as a reservoir for potential zoonotic pathogens and antibiotic resistant bacterial strains. In order to describe the feline gastrointestinal microbial diversity, fecal samples from cats have been characterized using both culture-dependent and culture-independent methods. However, data correlating total microbial composition and their functions are lacking. Present descriptive study evaluated both phylogenetic and metabolic diversity of the feline intestinal microbiota using GS Junior titanium shotgun pyrosequencing. A total of 152,494 pyrosequencing reads (5405 assembled contigs) were generated and classified into both phylogenetic and metabolic profiles of the feline intestinal microbiota. The Bacteroides/Chlorobi group was the most predominant bacterial phylum comprising ~68% of total classified diversity, followed by Firmicutes (~13%) and Proteobacteria (~6%) respectively. Archaea, fungi and viruses made up the minor communities in the overall microbial diversity. Interestingly, this study also identified a range of potential enteric zoonotic pathogens (0.02–1.25%) and genes involved in antimicrobial resistance (0.02–0.7%) in feline fecal materials. Based on clustering among nine gastrointestinal metagenomes from five different monogastric hosts (dog, human, mice, cat and chicken), the cat metagenome clustered closely together with chicken in both phylogenetic and metabolic level (>80%). Future studies are required to provide deeper understandings on both intrinsic and extrinsic effects such as impact of age, genetics and dietary interventions on the composition of the feline gastrointestinal microbiome. ► We described the gene-centric metagenomics analysis of feline intestinal microbiota. ► Comparisons were made with available metagenomes from other species. ► Potential pathogens and antimicrobial resistance genes were identified.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2012.01.001