Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids

Different species of camelids play an important role in the epidemiology of various emerging infectious diseases such as Middle East respiratory syndrome. For precise investigations of the immunopathogenesis in these host species, appropriate immunohistochemical markers are highly needed in order to...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2017-02, Vol.184, p.42-53
Main Authors: Uhde, Ann-Kathrin, Lehmbecker, Annika, Baumgärtner, Wolfgang, Spitzbarth, Ingo
Format: Article
Language:English
Subjects:
Alpaca
Animals
Antibodies - immunology
B cells
Bactrian
Camelids
Camelids, New World - anatomy & histology
Camelids, New World - immunology
Camelus - anatomy & histology
Camelus - immunology
Cross Reactions - immunology
Dromedary
Immunohistochemistry
Llama
Lymphoid Tissue - anatomy & histology
Lymphoid Tissue - immunology
Macrophages
Paraffin Embedding - veterinary
T cells
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Summary:Different species of camelids play an important role in the epidemiology of various emerging infectious diseases such as Middle East respiratory syndrome. For precise investigations of the immunopathogenesis in these host species, appropriate immunohistochemical markers are highly needed in order to phenotype distinct immune cells populations in camelids. So far, specific immunohistochemical markers for camelid immune cells are rarely commercially available, and cross-reactivity studies are restricted to the use of frozen dromedary tissues. To bridge this gap, 14 commercially available primary antibodies were tested for their suitability to demonstrate immune cell populations on formalin fixed paraffin-embedded (FFPE) tissue sections of dromedaries, Bactrian camels, llamas, and alpacas in the present study. Out of these, 9 antibodies directed against CD3, CD20, CD79α, HLA-DR, Iba-1, myeloid/histiocyte antigen, CD204, CD208, and CD68 antigen exhibited distinct immunoreaction patterns to certain camelid immune cell subsets. The distribution of these antigens was comparatively evaluated in different anatomical compartments of thymus, spleen, mesenteric, and tracheobronchial lymph nodes. The presented results will provide a basis for further investigations in camelids, especially with respect to the role of the immune response in certain infectious diseases, which harbor a considerable risk to spill over to other species.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2017.01.001