Antigenic Cross-Reactivity between Severe Acute Respiratory Syndrome—Associated Coronavirus and Human Coronaviruses 229E and OC43

Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndr...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-06, Vol.191 (12), p.2033-2037
Main Authors: Che, Xiao-yan, Qiu, Li-wen, Liao, Zhi-yong, Wang, Ya-di, Wen, Kun, Pan, Yu-xian, Hao, Wei, Mei, Ya-bo, Cheng, Vincent C. C., Yuen, Kwok-yung
Format: Article
Language:English
Subjects:
Adult
Animals
Antibodies
Antibodies, Viral - blood
Antigens, Viral - blood
Biological and medical sciences
Coronavirus
Coronavirus 229E, Human - immunology
Coronavirus OC43, Human - immunology
Cross reaction
Cross Reactions
Enzyme linked immunosorbent assay
Female
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Infectious diseases
Major and Brief Reports
Male
Medical sciences
Microbiology
Miscellaneous
Monoclonal antibodies
Nucleocapsid
Nucleocapsid proteins
SARS
SARS coronavirus
SARS virus
SARS Virus - immunology
Severe acute respiratory syndrome
Virology
Viruses
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Summary:Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a ⩾4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.
ISSN:0022-1899
1537-6613
DOI:10.1086/430355