Towards a better testicular sperm extraction: novel sperm sorting technologies for non-motile sperm extracted by microdissection TESE

Non-obstructive azoospermia (NOA) is the most severe form of male factor infertility. It is characterized by a lack of spermatogenesis in the seminiferous tubules. Microdissection testicular sperm extraction (microTESE) has significantly improved testicular sperm retrieval rates compared to conventi...

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Bibliographic Details
Published in:	Translational andrology and urology 2020-03, Vol.9 (Suppl 2), p.S206-S214
Main Authors:	Mangum, Cathy L, Patel, Darshan P, Jafek, Alexander R, Samuel, Raheel, Jenkins, Tim G, Aston, Kenneth I, Gale, Bruce K, Hotaling, James M
Format:	Article
Language:	eng
Subjects:	Review
Online Access:	Get full text
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Summary:	Non-obstructive azoospermia (NOA) is the most severe form of male factor infertility. It is characterized by a lack of spermatogenesis in the seminiferous tubules. Microdissection testicular sperm extraction (microTESE) has significantly improved testicular sperm retrieval rates compared to conventional techniques for NOA. Following testicular biopsy, the sperm is usually non-motile and contained within seminiferous tubules requiring extensive laboratory processing to find individual sperm sufficient for artificial reproductive technologies (ART). Current techniques include mechanical and enzymatic processing which is time-consuming and often damaging to sperm. We review novel techniques that may help improve sperm retrieval rates after microTESE including microfluidics (dielectrophoretic cell sorting, spiral channel sorting, and pinched flow fractionation), fluorescence-activated cell sorting (FACS), and magnetic-activated cell sorting (MACS).
ISSN:	2223-4691 2223-4683 2223-4691