Equine immunoglobulin F(ab')2 fragments protect cats against feline calicivirus infection

Feline calicivirus (FCV) causes upper respiratory tract infections in felines and threatens the health of wild and domestic felines. Clinically, specific drugs to treat FCV have not yet been developed. Here, IgG was extracted from inactivated FCV-immunized horse sera. Equine F(ab')2 fragments w...

Full description

Saved in:
Bibliographic Details
Published in:International immunopharmacology 2019-10, Vol.75, p.105714-105714, Article 105714
Main Authors: Cui, Zhanding, Li, Dengliang, Yi, Shushuai, Guo, Yanbing, Dong, Guoying, Niu, Jiangting, Zhao, Han, Zhang, Ying, Zhang, Shengnan, Cao, Lili, Wang, Kai, Zhao, Yongkun, Hu, Guixue
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - immunology
Caliciviridae Infections - therapy
Caliciviridae Infections - veterinary
Caliciviridae Infections - virology
Calicivirus, Feline - immunology
Cat
Cat Diseases - therapy
Cat Diseases - virology
Cats
Chemical compounds
Column chromatography
Equine
F(ab')2 fragment
FCV
Female
Fragments
Health risks
Horses
Horses - immunology
Immunization
Immunization, Passive
Immunoglobulin Fab Fragments - therapeutic use
Immunoglobulin G
Immunoglobulin G - immunology
Immunoglobulins
Infections
Lung - virology
Pepsin
Pharmacology
Respiratory tract
Respiratory tract diseases
Signs and symptoms
Spleen
Spleen - virology
Trachea
Trachea - virology
Viral Vaccines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Feline calicivirus (FCV) causes upper respiratory tract infections in felines and threatens the health of wild and domestic felines. Clinically, specific drugs to treat FCV have not yet been developed. Here, IgG was extracted from inactivated FCV-immunized horse sera. Equine F(ab')2 fragments were obtained from pepsin-digested IgG and then purified by protein-G column chromatography. In our study, equine immunoglobulin F(ab')2 fragments showed efficient neutralizing activity in vitro against FCV and had therapeutic and prophylactic effects in FCV-infected cats. The anti-FCV-specific F(ab')2 fragment can significantly alleviate the clinical symptoms of FCV-infected cats and reduce the viral loads of the trachea, lung and spleen. These results indicate that the F(ab')2 fragment prepared from inactivated FCV-immunized horses may be used as a prophylactic and therapeutic agent for diseases caused by FCV. •High-purity anti-FCV F(ab')2 fragments were prepared from equine IgG.•F(ab')2 fragments can bind to FCV both in vivo and in vitro.•F(ab')2 fragments can reduce the clinical symptoms in kittens infected with FCV.•Passive transfer of equine immune antibodies significantly reduced virus titers in the lungs and trachea of kittens.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2019.105714