Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein

To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2 + SARS-recovered donors at different time points post disease. CD8 +DimerX-S1...

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Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2004-11, Vol.113 (2), p.151-154
Main Authors: Wang, Yue-Dan, Chen, Wei Feng
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytometry
DimerX
ELISPOT
Epitopes, T-Lymphocyte - immunology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
HLA-A2
HLA-A2 Antigen - immunology
Humans
Immune response
Immunoglobulins - immunology
Immunopathology
Interferon-gamma - immunology
Iothalamic Acid - analogs & derivatives
Medical sciences
Meglumine
SARS
SARS coronavirus
SARS Virus - immunology
Severe Acute Respiratory Syndrome - immunology
T-Lymphocytes, Cytotoxic - immunology
Viral Fusion Proteins
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Summary:To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2 + SARS-recovered donors at different time points post disease. CD8 +DimerX-S1203 + CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8 +DimerX-S1203 + cells paralleled the numbers of interferon-γ-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus.
ISSN:1521-6616
1521-7035
DOI:10.1016/j.clim.2004.07.004