Transcriptomic signature of gut microbiome-contacting cells in colon of spontaneously hypertensive rats

Fecal matter transfer from hypertensive patients and animals into normotensive animals increases blood pressure, strengthening the evidence for gut-microbiota interactions in the control of blood pressure. However, cellular and molecular events involved in gut dysbiosis-associated hypertension remai...

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Bibliographic Details
Published in:Physiological genomics 2020-03, Vol.52 (3), p.121-132
Main Authors: Yang, Tao, Li, Hongbao, Oliveira, Aline C, Goel, Ruby, Richards, Elaine M, Pepine, Carl J, Raizada, Mohan K
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - metabolism
Alkaline phosphatase
Alkaline Phosphatase - metabolism
Animals
Blood Pressure
CD3 antigen
CD3 Complex - metabolism
Cell surface
Colon
Colon - metabolism
Cytokines - metabolism
Digestive system
Digestive tract
Dysbacteriosis
Dysbiosis
Epithelial cells
Epithelium
Gastrointestinal Microbiome - genetics
Gene expression
Hypertension
Hypertension - metabolism
Interleukin 1
Interleukin 12 receptors
Interleukin 16
Interleukin 7
Intestinal microflora
Intestinal Mucosa - metabolism
Intestine
Isoenzymes - metabolism
Lymphocytes T
Male
Microbiomes
Microbiota
Phosphoproteins - metabolism
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Receptors, Antigen, T-Cell - metabolism
RNA-Seq
T cell receptors
Transcriptome
Transforming growth factor-b1
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Summary:Fecal matter transfer from hypertensive patients and animals into normotensive animals increases blood pressure, strengthening the evidence for gut-microbiota interactions in the control of blood pressure. However, cellular and molecular events involved in gut dysbiosis-associated hypertension remain poorly understood. Therefore, our objective in this study was to use gene expression profiling to characterize the gut epithelium layer in the colon in hypertension. We observed significant suppression of components of T cell receptor (TCR) signaling in the colonic epithelium of spontaneously hypertensive rats (SHR) when compared with Wistar Kyoto (WKY) normotensive rats. Western blot analysis confirmed lower expression of key proteins including T cell surface glycoprotein CD3 gamma chain (Cd3g) and lymphocyte cytosolic protein 2 (Lcp2). Furthermore, lower expression of cytokines and receptors responsible for lymphocyte proliferation, differentiation, and activation (e.g., , , , , ) was observed in the colonic epithelium of the SHR. Finally, and its product, intestinal alkaline phosphatase, primarily localized in the epithelial cells, were profoundly lower in the SHR. These observations demonstrate that the colonic epithelium undergoes functional changes linked to altered immune, barrier function, and dysbiosis in hypertension.
ISSN:1094-8341
1531-2267
DOI:10.1152/physiolgenomics.00087.2019