Transcriptomic analysis of female and male gonads in juvenile snakeskin gourami (Trichopodus pectoralis)

The snakeskin gourami (Trichopodus pectoralis) exhibits sexual dimorphism, particularly in body size. Since the snakeskin gourami is usually marketed during sexual maturation, the sexual size dimorphism has become an economically important trait. Sex-biased gene expression plays a key role in phenot...

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Bibliographic Details
Published in:Scientific reports 2020-03, Vol.10 (1), p.5240-5240, Article 5240
Main Authors: Boonanuntanasarn, Surintorn, Jangprai, Araya, Na-Nakorn, Uthairat
Format: Article
Language:English
Subjects:
Animals
Body size
Female
Fish Proteins - genetics
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Ontology
Gonads
Male
Ovary - cytology
Ovary - physiology
p53 Protein
Perciformes - genetics
Polymerase chain reaction
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Reverse transcription
Ribonucleic acid
RNA
Sex Characteristics
Sex differentiation
Sexual dimorphism
Sexual Maturation
Testis - cytology
Testis - physiology
Transcriptomics
Trichopodus pectoralis
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Summary:The snakeskin gourami (Trichopodus pectoralis) exhibits sexual dimorphism, particularly in body size. Since the snakeskin gourami is usually marketed during sexual maturation, the sexual size dimorphism has become an economically important trait. Sex-biased gene expression plays a key role in phenotypic sexual dimorphism. Therefore, using high-throughput RNA sequencing (RNA-seq) technology, we aimed to explore the differentially expressed genes (DEGs) in ovary and testis during sex differentiation in juvenile snakeskin gourami. Our results revealed a number of DEGs were demonstrated to be overexpressed in ovary (11,625 unigenes) and testis (16,120 unigenes), and the top 10 female-biased (rdh7, dnajc25, ap1s3, zp4, polb, parp12, trim39, gucy2g, rtbs, and fdxr) and male-biased (vamp3, nbl1, dnah2, ccdc11, nr2e3, spats1, pih1d2, tekt3, fbxo36, and mybl2) DEGs were suggested to be mainly associated with ovary and testis differentiation, respectively. Additionally, using real-time reverse transcription polymerase chain reaction (qRT-PCR), validation of the differential expression of 21 genes that were previously shown to be related to gonad development was performed (ar, bHLH, cyp19a1, daz, dead-end, esrb, esrrg, gnrhr, gpa, gsg1l, hsd17B, mospd1, nanos-1, nanos-2, p53, piwi-1, piwi-2, rerg, rps6ka, tgf-beta, and VgR). The results showed a significantly positive correlation (0.84; P 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-61738-0