Inhibition of Staphylococcus aureus PriA Helicase by Flavonol Kaempferol

Staphylococcus aureus is an important etiological agent responsible for healthcare-associated infections. In this study, the effect of flavonoids on the inhibition of S. aureus PriA (SaPriA), an essential helicase for DNA replication restart, which is critical for bacterial survival, was investigate...

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Bibliographic Details
Published in:Protein Journal 2015-06, Vol.34 (3), p.169-172
Main Authors: Huang, Yen-Hua, Huang, Chien-Chih, Chen, Cheng-Chieh, Yang, Kai-Jr, Huang, Cheng-Yang
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Animal Anatomy
Bacterial Proteins - antagonists & inhibitors
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Biochemistry
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
DNA Helicases - antagonists & inhibitors
DNA Helicases - chemistry
DNA Helicases - metabolism
DNA replication
Flavonoids - pharmacology
Health aspects
Histology
Hydrolysis
Isoflavones
Kaempferols - pharmacology
Morphology
Organic Chemistry
Staphylococcus aureus
Staphylococcus aureus - enzymology
Staphylococcus aureus infections
Staphylococcus infections
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Summary:Staphylococcus aureus is an important etiological agent responsible for healthcare-associated infections. In this study, the effect of flavonoids on the inhibition of S. aureus PriA (SaPriA), an essential helicase for DNA replication restart, which is critical for bacterial survival, was investigated. Using vanadate-sensitive colorimetric assay, the concentration of phosphate, from ATP hydrolysis by SaPriA, was decreased to 37 and 69 %, respectively, in the presence of 35 μM kaempferol and myricetin. The effect of quercetin, galangin, dihydromyricetin, and myricitrin was insignificant. From titration curve, IC 50 of kaempferol for SaPriA was determined to be 22 ± 2 μM. Using fluorescence quenching, we identified that kaempferol can bind to SaPriA with K d of 9.1 ± 3.2 μM. To our knowledge, these preliminary results constituted the first study regarding that naturally occurring product such as flavonols kaempferol and myricetin can be potent inhibitors targeting PriA.
ISSN:1572-3887
1573-4943
1875-8355
DOI:10.1007/s10930-015-9609-y